000			nab a22 7a 4500
999			_c62315 _d62307
001			62315
003			MX-TxCIM
005			20200724231622.0
008			200124s2016 xxu|||p|op||| 00| 0 eng d
022			_a0036-8075
022			_a 1095-9203 (Online)
024	8		_ahttps://doi.org/10.1126/science.aaf2091
040			_aMX-TxCIM
041			_aeng
100	1		_914780 _aBrown, K.A.
245	1	0	_aLight-driven dinitrogen reduction catalyzed by a CdS : _bnitrogenase MoFe protein biohybrid
260			_aWashington, DC (USA) : _bAAAS, _c2016.
500			_aPeer review
520			_aThe splitting of dinitrogen (N2) and reduction to ammonia (NH3) is a kinetically complex and energetically challenging multistep reaction. In the Haber-Bosch process, N2 reduction is accomplished at high temperature and pressure, whereas N2 fixation by the enzyme nitrogenase occurs under ambient conditions using chemical energy from adenosine 5′-triphosphate (ATP) hydrolysis. We show that cadmium sulfide (CdS) nanocrystals can be used to photosensitize the nitrogenase molybdenum-iron (MoFe) protein, where light harvesting replaces ATP hydrolysis to drive the enzymatic reduction of N2 into NH3. The turnover rate was 75 per minute, 63% of the ATP-coupled reaction rate for the nitrogenase complex under optimal conditions. Inhibitors of nitrogenase (i.e., acetylene, carbon monoxide, and dihydrogen) suppressed N2 reduction. The CdS:MoFe protein biohybrids provide a photochemical model for achieving light-driven N2 reduction to NH3.
546			_aText in English
650		7	_2AGROVOC _92912 _aNitrogen
650		7	_2AGROVOC _914781 _aNitrogenase
650		7	_2AGROVOC _98925 _aMolybdenum
650		7	_2AGROVOC _914782 _aCadmium
700	1		_914783 _aHarris, D.F.
700	1		_914784 _aWilker, M.B.
700	1		_914785 _aRasmussen, A.
700	1		_914786 _aKhadka, N.
700	1		_914787 _aHamby, H.
700	1		_914788 _aKeable, S.
700	1		_914789 _aDukovic, G.
700	1		_914790 _aPeters, J.W.
700	1		_914791 _aSeefeldt, L.C.
700	1		_914792 _aKing, P.W.
773	0		_dWashington, DC (USA) : AAAS, 2016. _gv. 352 no. 6284, p. 448-450 _tScience _x 0036-8075 _wu444126
942			_2ddc _cJA _n0