000 | nab a22 7a 4500 | ||
---|---|---|---|
999 |
_c62291 _d62283 |
||
001 | 62291 | ||
003 | MX-TxCIM | ||
005 | 20200724225504.0 | ||
008 | 200124s2004 ne |||p|op||| 00| 0 eng d | ||
022 | _a0929-1873 | ||
022 | _a1573-8469 (Online) | ||
024 | 8 | _ahttps://doi.org/10.1023/B:EJPP.0000019791.81935.6d | |
040 | _aMX-TxCIM | ||
041 | _aeng | ||
100 | 1 |
_914727 _aJiménez-Hidalgo, I. |
|
245 | 1 | 0 | _aIdentification and characterisation of bacteria causing soft-rot in Agave tequilana |
260 |
_aDordrecht (Netherlands) : _bSpringer, _c2004. |
||
500 | _aPeer review | ||
520 | _aAgave tequilana is the raw material for the production of the alcoholic beverage tequila. A bacterial disease has affected the A. tequilana crop in recent years. Previous reports based on colony and cell morphology, Gram stain and potato rot indicated that Erwinia sp. is the main pathogen. We isolated a several bacterial isolates capable of producing soft-rot symptoms in greenhouse pathogenicity assays. An extensive characterisation involving pathogenicity tests, fatty acid profile, metabolic and physiological properties, ribosomal DNA sequence and intergenic transcribed spacer amplification (ITS-PCR) and restriction banding pattern (ITS-RFLP) was made of each isolate. Three different species: Erwinia cacticida, Pantoea agglomerans and Pseudomonas sp. were identified. Fatty acid and metabolic profiles gave low similarity values of identification but 16S rDNA sequence, ITS-PCR and ITS-RFLP confirmed the identification of E. cacticida. In the phylogenetic tree, E. cacticida from blue agave was grouped neither with E. cacticida type strains nor with Erwinia carotovora. This is the first report that associates E. cacticida with A. tequilana soft-rot symptoms. | ||
546 | _aText in English | ||
650 | 7 |
_2AGROVOC _914728 _aAgave tequilana |
|
650 | 7 |
_2AGROVOC _99519 _aErwinia |
|
650 | 7 |
_2AGROVOC _914729 _aPantoea |
|
650 | 7 |
_2AGROVOC _99520 _aPseudomonas |
|
650 | 7 |
_2AGROVOC _97951 _aRots |
|
700 | 1 |
_914730 _aVirgen-Calleros, G. |
|
700 | 1 |
_914731 _aMartínez-de la Vega, O. |
|
700 | 1 |
_914732 _aVandemark, G. |
|
700 | 1 |
_914733 _aOlalde-Portugal, V. |
|
773 | 0 |
_dDordrecht (Netherlands) : Springer, 2004. _gv. 110, no. 3, p. 317-331 _tEuropean Journal of Plant Pathology _wu445058 _x0929-1873 |
|
942 |
_2ddc _cJA _n0 |