000 nab a22 7a 4500
999 _c62291
_d62283
001 62291
003 MX-TxCIM
005 20200724225504.0
008 200124s2004 ne |||p|op||| 00| 0 eng d
022 _a0929-1873
022 _a1573-8469 (Online)
024 8 _ahttps://doi.org/10.1023/B:EJPP.0000019791.81935.6d
040 _aMX-TxCIM
041 _aeng
100 1 _914727
_aJiménez-Hidalgo, I.
245 1 0 _aIdentification and characterisation of bacteria causing soft-rot in Agave tequilana
260 _aDordrecht (Netherlands) :
_bSpringer,
_c2004.
500 _aPeer review
520 _aAgave tequilana is the raw material for the production of the alcoholic beverage tequila. A bacterial disease has affected the A. tequilana crop in recent years. Previous reports based on colony and cell morphology, Gram stain and potato rot indicated that Erwinia sp. is the main pathogen. We isolated a several bacterial isolates capable of producing soft-rot symptoms in greenhouse pathogenicity assays. An extensive characterisation involving pathogenicity tests, fatty acid profile, metabolic and physiological properties, ribosomal DNA sequence and intergenic transcribed spacer amplification (ITS-PCR) and restriction banding pattern (ITS-RFLP) was made of each isolate. Three different species: Erwinia cacticida, Pantoea agglomerans and Pseudomonas sp. were identified. Fatty acid and metabolic profiles gave low similarity values of identification but 16S rDNA sequence, ITS-PCR and ITS-RFLP confirmed the identification of E. cacticida. In the phylogenetic tree, E. cacticida from blue agave was grouped neither with E. cacticida type strains nor with Erwinia carotovora. This is the first report that associates E. cacticida with A. tequilana soft-rot symptoms.
546 _aText in English
650 7 _2AGROVOC
_914728
_aAgave tequilana
650 7 _2AGROVOC
_99519
_aErwinia
650 7 _2AGROVOC
_914729
_aPantoea
650 7 _2AGROVOC
_99520
_aPseudomonas
650 7 _2AGROVOC
_97951
_aRots
700 1 _914730
_aVirgen-Calleros, G.
700 1 _914731
_aMartínez-de la Vega, O.
700 1 _914732
_aVandemark, G.
700 1 _914733
_aOlalde-Portugal, V.
773 0 _dDordrecht (Netherlands) : Springer, 2004.
_gv. 110, no. 3, p. 317-331
_tEuropean Journal of Plant Pathology
_wu445058
_x0929-1873
942 _2ddc
_cJA
_n0