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999			_c62222 _d62214
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003			MX-TxCIM
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008			200325s2015||||xxk|||p|op||||00||0|eng|d
022			_a0706-0661
022			_a1715-2992 (Online)
024	8		_ahttps://doi.org/10.1080/07060661.2015.1113442
040			_aMX-TxCIM
041			_aeng
100	1		_aTambong, J.T. _914567
245	1	0	_aSpecific identification and detection of Pantoea stewartii subsp. stewartii using a membrane-based multi-gene oligonucleotide array
260			_aUnited Kingdom : _bTaylor and Francis, _c2015.
500			_aPeer review
520			_aThe Stewart’s wilt pathogen (Pantoea stewartii subsp. stewartii; Pss) of sweet corn is classified as a quarantine bacterium, requiring certification of grain shipments made to over 60 countries. This study reports the development and validation of the first multi-gene array for accurate and specific differentiation of Pss from Pantoea stewartii subsp. indologenes (Psi) and other Pantoea species using DNA, or RNA after generation of cDNA. The technique consisted of multiplex (16S rRNA, leuS, gyrB, rpoB, cpsD) PCR amplifications using universal and specific primers in a single reaction, followed by the hybridization of the digoxigenin-labelled amplicons to 22 specific oligonucleotide probes (19- to 24-mers) immobilized on a nylon membrane. The sensitivity of the array (10 fg of DNA) compared favourably with TaqMan real-time PCR. The specificity and reliability of the array was tested on 65 bacterial strains consisting of Pantoea species, closely and distantly related bacterial genera. Specific detection of Pss in infected corn leaves and seed homogenates was also validated using growth chamber-inoculated plants. The reported multi-gene DNA array could be a reliable tool for routine and specific detection of Pss.
546			_aText in English
650		7	_aMaize _gAGROVOC _2 _91173
650		7	_2AGROVOC _914568 _aPantoea stewartii
773	0		_tCanadian Journal of Plant Pathology _gv. 37, no. 4, p. 414-426 _dUnited Kingdom : Taylor and Francis, 2015. _x0706-0661 _wu444208
942			_cJA _n0 _2ddc