000			nab a22 7a 4500
999			_c62138 _d62130
001			62138
003			MX-TxCIM
005			20200629211301.0
008			200618s2020 ne |||p|op||| 00| 0 eng d
022			_a0981-9428
024	8		_ahttps://doi.org/10.1016/j.plaphy.2020.04.013
040			_aMX-TxCIM
041			_aeng
100	0		_914269 _aHaidong Yu
245	1	0	_aTowards identification of molecular mechanism in which the overexpression of wheat cytosolic and plastid glutamine synthetases in tobacco enhanced drought tolerance
260			_aAmsterdam (Netherlands) : _bElsevier, _c2020.
500			_aPeer review
520			_aGlutamine synthetases (GS) play an essential role in Nitrogen assimilation. Nonetheless, information respecting the molecular functions of GS in drought tolerance (DT) is limited. Here we show that overexpressing cytosolic GS1 or plastidic GS2 gene in tobacco enhanced DT of both root and leaf tissues of the two transgenic seedlings (named as GS1-TR and GS2-TR). RNA-seq analysis on root tissues showed that 83 aquaporin (AQP) genes were identified. Among them, 37 differential expression genes (DEGs) were found in the GS1-TR roots under normal condition, and all were down-regulated; no any DEGs in the GS2-TR roots were found. Contrastingly, under drought, 28 and 32 DEGs of AQP were up-regulated in GS1-TR and GS2-TR roots, respectively. GC-MS analysis on leaf tissues showed that glutamine (Gln) concentrations were negatively correlated AQP expressions in the all four conditions, which suggests that Gln, as a signal molecule, can negatively regulate many AQP expressions. Prestress accumulation of sucrose and proline (Pro) and chlorophyll, and had higher activities of ROS scavengers also contribute the plant DT in both of the two transgenic plants under drought. In addition, 5-aminolevulinic acid (ALA) was up-accumulated in GS2-TR leaves solely under normal condition, which leads to its net photosynthetic rate higher than that in GS1-TR leaves. Last but not the less, the PYL-PP2C-SnRK2 core ABA-signaling pathway was uniquely activated in GS1-TR independent of drought stress (DS). Therefore, our results suggest a possible model reflecting how overexpression of wheat TaGS1 and TaGS2 regulate plant responses to drought.
546			_aText in English
650		7	_2AGROVOC _914270 _aGlutamate ammonia ligase
650		7	_2AGROVOC _914271 _aGlutamine
650		7	_2AGROVOC _96733 _aNitrogen metabolism
650		7	_2AGROVOC _93517 _aABA
650		7	_2AGROVOC _91082 _aDrought tolerance
700	0		_914272 _aYiming Zhang
700	0		_914273 _aZhiyong Zhang
700	0		_914274 _aJie Zhang
700	0		_914275 _aYihao Wei
700	0		_914276 _aXiting Jia
700	0		_914277 _aXiaochun Wang
700	0		_914278 _aXinming Ma
773	0		_dAmsterdam (Netherlands) : Elsevier, 2020. _gv. 151, p. 608-620 _tPlant Physiology and Biochemistry _x0981-9428 _wu91708
942			_2ddc _cJA _n0