000			nab a22 7a 4500
999			_c62137 _d62129
001			62137
003			MX-TxCIM
005			20200629211223.0
008			200618s2020 xxk|||p|op||| 00| 0 eng d
022			_a0031-9317
022			_a1399-3054 (Online)
024	8		_ahttps://doi.org/10.1111/ppl.13102
040			_aMX-TxCIM
041			_aeng
100	1		_914264 _aShamloo-Dashtpagerdi, R.
245	1	0	_aPlausible association between drought stress tolerance of barley (Hordeum vulgare L.) and programmed cell death via MC1 and TSN1 genes
260			_aUnited Kingdom : _bWiley, _c2020.
500			_aPeer review
520			_aStudying the drought‐responsive transcriptome is of high interest as it can serve as a blueprint for stress adaptation strategies. Despite extensive studies in this area, there are still many details to be uncovered, such as the importance of each gene involved in the stress response as well as the relationship between these genes and the physiochemical processes governing stress tolerance. This study was designed to address such important details and to gain insights into molecular responses of barley (Hordeum vulgare L.) to drought stress. To that, we combined RNA‐seq data analysis with field and greenhouse drought experiments in a systems biology approach. RNA‐sequence analysis identified a total of 665 differentially expressed genes (DEGs) belonging to diverse functional categories. A gene network was derived from the DEGs, which comprised of a total of 131 nodes and 257 edges. Gene network topology analysis highlighted two programmed cell death (PCD) modulating genes, MC1 (metacaspase 1 ) and TSN1 (Tudor‐SN 1 ), as important (hub) genes in the predicted network. Based on the field trial, a drought‐tolerant and a drought‐susceptible barley genotype was identified from eight tested cultivars. Identified genotypes exhibited different physiochemical characteristics, including proline content, chlorophyll concentration, percentage of electrolyte leakage and malondialdehyde content as well as expression profiles of MC1 and TSN1 genes. Machine learning and correspondence analysis revealed a significant relationship between drought tolerance and measured characteristics in the context of PCD. Our study provides new insights which bridge barley drought tolerance to PCD through MC1 and TSN1 pathway.
546			_aText in English
650		7	_2AGROVOC _91081 _aDrought stress
650		7	_2AGROVOC _91082 _aDrought tolerance
650		7	_2AGROVOC _91018 _aBarley
650		7	_2AGROVOC _914265 _aCell death
650		7	_2AGROVOC _93563 _aGenes
700	1		_914266 _aLindlöf, A.
700	1		_914267 _aAliakbari, M.
700	1		_914268 _aPirasteh-Anosheh, H.
773	0		_dUnited Kingdom : Wiley, 2020. _gIn press _tPhysiologia Plantarum _x1399-3054 _wu444664
942			_2ddc _cJA _n0