000 02023nab a22003497a 4500
999 _c61978
_d61970
001 61978
003 MX-TxCIM
005 20211006072303.0
008 180103s2008 gw |||p|op||| 00| 0 eng d
022 _a0040-5752
022 _a1432-2242 (Online)
024 8 _ahttps://doi.org/10.1007/s00122-002-1048-4
040 _aMX-TxCIM
041 _aeng
100 _91406
_aEllis, M.
245 1 1 _a"Perfect" markers for the Rht-B1b and Rht-D1b dwarfing genes in wheat
260 _aBerlin (Germany) :
_bSpringer,
_c2002.
500 _aPeer review
520 _aPCR-based markers were developed to detect the point mutations responsible for the two major semi-dwarfing genes Rht-B1b (Rht1) and Rht-D1b (Rht2) in wheat. These markers were validated by testing 19 wheat varieties of known Rht genotype. They included Rht-B1b and Rht-D1b dwarfs, double-mutant varieties and tall wheats. These were correctly genotyped with the Rht-B1b and Rht-D1b-specific primers, as well as markers specific for the tall alleles Rht-B1a and Rht-D1a. Using a family of doubled-haploid lines segregating for Rht-B1b and Rht-D1b, the markers were mapped to the expected homoeologous regions of chromosomes 4B and 4D, respectively. Both markers were strongly correlated with a reduction in height, accounting for 23% (Rht-B1b) and 44% (Rht-D1b) of the phenotypic variance in the population. These markers will have utility in marker-assisted selection of the Rht-B1b and Rht-D1b genes in wheat breeding programs.
546 _aText in English
650 7 _aWheat
_gAGROVOC
_2
_91310
650 7 _911189
_aHeight
_2AGROVOC
650 7 _93055
_aDwarfism
_2AGROVOC
650 7 _912563
_aPCR
_2AGROVOC
650 7 _91848
_aGenetic markers
_2AGROVOC
700 1 _92079
_aSpielmeyer, W.
700 1 _913592
_aGale, K.
700 1 _9667
_aRebetzke, G.J.
700 1 _910734
_aRichards, R.A.
773 0 _gv. 105, no. 6-7, p. 1038-1042
_tTheoretical and Applied Genetics
_x0040-5752
_dBerlin (Germany) : Springer, 2002.
_wu444762
942 _2ddc
_cJA
_n0