000			02448nab a22003737a 4500
999			_c61955 _d61947
001			61955
003			MX-TxCIM
005			20200601164302.0
008			180103s1999 xxk|||p|op||| 00| 0 eng d
022			_a0960-2585
022			_a1475-2735 (Online)
024	8		_ahttps://doi.org/10.1017/S0960258599000094
040			_aMX-TxCIM
041			_aeng
100	1		_913506 _aPortis, E.
245	1	0	_aMolecular and physiological markers during seed development of peppers (Capsicum annuum L.) : _bDNA replication and β-tubulin synthesis
260			_aCambridge (United Kingdom) : _bCambridge University Press, _c1999.
500			_aPeer review
520			_aThe correlation between physiological markers for seed maturity (acquisition of germinability, definitive morphological traits and desiccation tolerance) and timing of the shutdown of nuclear replication activity and β-tubulin synthesis has been investigated in developing pepper seeds. Nuclear replication activity was analysed by flow cytometry. Expression of β-tubulin, a cell-cycle related structural protein, was detected in western blots of total protein extracts. In pepper embryos, DNA synthesis stopped 42–49 days after anthesis (DAA), at which time seeds started to acquire desiccation tolerance. A clear β-tubulin signal was detected up to 63 DAA, only 1 week before the acquisition of complete desiccation tolerance. Both nuclear replication and β-tubulin synthesis can therefore be used as markers for the physiological stage reached by developing pepper seeds. Detection of β-tubulin signals in embryonic tissues can also be helpful in discriminating immature seeds within a commercial seed lot.
546			_aText in English
650		7	_913507 _aDNA replication _2AGROVOC
650		7	_912754 _aCapsicum annuum _2AGROVOC
650		7	_97755 _aSeed development _2AGROVOC
700	1		_913508 _aMarzachì, C.
700	1		_913509 _aQuagliotti, L.
700	1		_913510 _aLanteri, S.
773	0		_gv. 9, no. 1, p. 85-90 _tSeed Science Research _x0960-2585 _dCambridge (United Kingdom) : Cambridge University Press, 1999. _wu96711
942			_2ddc _cJA _n0