| 000 | 02448nab a22003737a 4500 | ||
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| 999 |
_c61844 _d61836 |
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| 001 | 61844 | ||
| 003 | MX-TxCIM | ||
| 005 | 20200518221750.0 | ||
| 008 | 180103s2012 at |||p|op||| 00| 0 eng d | ||
| 022 | _a1445-4408 | ||
| 022 | _a1445-4416 (Online) | ||
| 024 | 8 | _ahttps://doi.org/10.1071/FP12143 | |
| 040 | _aMX-TxCIM | ||
| 041 | _aeng | ||
| 100 | 0 |
_912998 _aTong Wang |
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| 245 | 1 | 0 | _aTranscriptome identification of the resistance-associated genes (RAGs) to Aspergillus flavus infection in pre-harvested peanut (Arachis hypogaea) |
| 260 |
_aVictoria (Australia) : _bCSIRO Publising, _c2012. |
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| 500 | _aPeer review | ||
| 520 | _aPre-harvest aflatoxin contamination caused by Aspergillus favus is a major concern in peanut. However, little is known about the resistance mechanism, so the incorporation of resistance into cultivars with commercially-acceptable genetic background has been slowed. To identify resistance-associated genes potentially underlying the resistance mechanism, we compared transcriptome profiles in resistant and susceptible peanut genotypes under three different treatments: well watered, drought stress and both A. flavus and drought stress using a customised NimbleGen microarray representing 36 158 unigenes. Results showed that the profile of differentially expressed genes (DEGs) displayed a similar pattern of distribution among the functional classes between resistant and susceptible peanuts in response to drought stress. Under A. flavus infection with drought stress, a total of 490 unigenes involved in 26 pathways were differentially expressed in the resistant genotype YJ1 uniquely responding to A. flavus infection, in which 96 DEGs were related to eight pathways: oxidation reduction, proteolysis metabolism, coenzyme A biosynthesis, defence response, signalling, oligopeptide transport, transmembrane transport and carbohydrate biosynthesis/metabolism. Pathway analysis based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) database showed that eight networks were significantly associated with resistance to A. flavus infection in resistant genotype YJ1 compared with susceptible Yueyou7. To validate microarray analysis, 15 genes were randomly selected for real-time RT–PCR analysis. The results provided in this study may enhance our understanding of the pre-harvest peanut–A. flavus interaction and facilitate to develop aflatoxin resistant peanut lines in future breeding programs. | ||
| 546 | _aText in English | ||
| 650 | 7 |
_95637 _aAflatoxins _2AGROVOC |
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| 650 | 7 |
_92338 _aGroundnuts _2AGROVOC |
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| 650 | 7 |
_92277 _aGenetic resistance _2AGROVOC |
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| 700 | 0 |
_912999 _aXiao-Ping Chen |
|
| 700 | 0 |
_913000 _aHai-Fen Li |
|
| 700 | 0 |
_913001 _aHai-Yan Liu |
|
| 700 | 0 |
_913002 _aYan-Bin Hong |
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| 700 | 0 |
_913003 _aQing-Li Yang |
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| 700 | 0 |
_913004 _aXiao-Yuan Chi |
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| 700 | 0 |
_913005 _aZhen Yang |
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| 700 | 0 |
_913006 _aShan-Lin Yu |
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| 700 | 0 |
_913007 _aLing Li |
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| 700 | 0 |
_913008 _aXuan-Qiang Liang |
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| 773 | 0 |
_gv. 40, no. 3, p. 292-303 _tFunctional Plant Biology _x1445-4408 _dVictoria (Australia) : CSIRO Publising, 2012. _w447878 |
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| 942 |
_2ddc _cJA _n0 |
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