| 000 | 02409nab|a22003257a|4500 | ||
|---|---|---|---|
| 999 |
_c61800 _d61792 |
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| 001 | 61800 | ||
| 003 | MX-TxCIM | ||
| 005 | 20250923171408.0 | ||
| 008 | 200503s2012||||ne |||p|op||||00||0|eng|d | ||
| 022 | _a1380-3743 | ||
| 022 | _a1572-9788 (Online) | ||
| 024 | 8 | _ahttps://doi.org/10.1007/s11032-011-9566-x | |
| 040 | _aMX-TxCIM | ||
| 041 | _aeng | ||
| 100 | 0 |
_aYuanhong Han _912797 |
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| 245 | 1 | 0 | _aHigh-resolution melting analysis for SNP genotyping and mapping in tetraploid alfalfa (Medicago sativa L.) |
| 260 |
_a Dordrecht (Netherlands) : _bSpringer, _c2012. |
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| 500 | _aPeer review | ||
| 500 | _aOpen Access | ||
| 520 | _aSingle nucleotide polymorphisms (SNPs) represent the most abundant type of genetic polymorphism in plant genomes. SNP markers are valuable tools for genetic analysis of complex traits of agronomic importance, linkage and association mapping, genome-wide selection, map-based cloning, and marker-assisted selection. Current challenges for SNP genotyping in polyploid outcrossing species include multiple alleles per loci and lack of high-throughput methods suitable for variant detection. In this study, we report on a high-resolution melting (HRM) analysis system for SNP genotyping and mapping in outcrossing tetraploid genotypes. The sensitivity and utility of this technology is demonstrated by identification of the parental genotypes and segregating progeny in six alfalfa populations based on unique melting curve profiles due to differences in allelic composition at one or multiple loci. HRM using a 384-well format is a fast, consistent, and efficient approach for SNP discovery and genotyping, useful in polyploid species with uncharacterized genomes. Possible applications of this method include variation discovery, analysis of candidate genes, genotyping for comparative and association mapping, and integration of genome-wide selection in breeding programs. | ||
| 546 | _aText in English | ||
| 650 | 0 |
_aMedicago sativa _gAGROVOC _938417 |
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| 650 | 7 |
_2AGROVOC _92084 _aChromosome mapping |
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| 650 | 0 |
_aSingle nucleotide polymorphisms _gAGROVOC _910805 |
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| 700 | 0 |
_912798 _aDong-Man Khu |
|
| 700 | 1 |
_912799 _aMonteros, M.J. |
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| 773 | 0 |
_tMolecular Breeding _gv. 29, no. 2, p. 489-501 _dDordrecht (Netherlands) : Springer, 2012. _x1380-3743 _wu78961 |
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| 856 | 4 |
_uhttps://doi.org/10.1007/s11032-011-9566-x _yClick here to access online |
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| 942 |
_cJA _n0 _2ddc |
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