000			00595nab|a22002177a|4500
999			_c61775 _d61767
001			61775
003			MX-TxCIM
005			20200925164639.0
008			200423s2012||||xxk|||p|op||||00||0|eng|d
022			_a0022-0299
022			_a1469-7629 (Online)
024	8		_ahttps://doi.org/10.1017/S002202991200026X
040			_aMX-TxCIM
041			_aeng
100	1		_912683 _aCremonesi, P.
245	1	0	_aIdentification of Clostridium beijerinckii, Cl. butyricum, Cl. sporogenes, Cl. tyrobutyricum isolated from silage, raw milk and hard cheese by a multiplex PCR assay
260			_aCambridge (United Kingdom) : _bCambridge University Press, _c2012.
500			_aPeer review
520			_aLate blowing, caused by the outgrowth of clostridial spores present in raw milk and originating from silage, can create considerable product loss, especially in the production of hard and semi-hard cheeses. The conventional method for the isolation of Clostridium spp. from cheeses with late-blowing symptoms is very complicated and the identification of isolates is problematic. The aim of this work was the development of a multiplex PCR method for the detection of the main dairy-related clostridia such as: Cl. beijerinckii, Cl. butyricum, Cl. sporogenes, Cl. tyrobutyricum. Samples derived from silage, raw milk and hard cheese were analysed by the most probable number (MPN) enumeration. Forty-four bacterial strains isolated from gas positive tubes were used to check the reliability of the multiplex PCR assay. The specificity of the primers was tested by individually analysing each primer pair and the primer pair combined in the multiplex PCR. It was interesting to note that the samples not identified by the multiplex PCR assay were amplified by V2–V3 16S rRNA primer pair and the sequencing revealed the aligned 16S rRNA sequences to be Paenibacillus and Bacillus spp. This new molecular assay provides a simple promising alternative to traditional microbiological methods for a rapid, sensitive detection of clostridia in dairy products.
546			_aText in English
650		7	_2AGROVOC _912684 _aClostridium
650		7	_2AGROVOC _912685 _aRaw milk
650		7	_2AGROVOC _910374 _aSilage
650		7	_2AGROVOC _98681 _aCheese
700	1		_912686 _aVanoni, L.
700	1		_912687 _aSilvetti, T.
700	1		_912688 _aMorandi, S.
700	1		_912689 _aBrasca, M.
773	0		_tJournal of Dairy Research _gv. 79, no. 3, p. 318-323 _dCambridge (United Kingdom) : Cambridge University Press, 2012. _x0022-0299 _wu76377
942			_cJA _n0 _2ddc