000			03232nab|a22003977a|4500
001			61625
003			MX-TxCIM
005			20231016185004.0
008			200331s2020||||sz |||p|op||||00||0|eng|d
022			_a2223-7747 (Online)
024	8		_ahttps://doi.org/10.3390/plants9040459
040			_aMX-TxCIM
041			_aeng
100	1		_aChaikam, V. _9936 _8INT3356 _gGlobal Maize Program
245	1	0	_aImproving the efficiency of colchicine-based chromosomal doubling of maize haploids
260			_aBasel (Switzerland) : _bMDPI, _c2020.
500			_aPeer review
500			_aOpen Access
520			_aProduction and use of doubled haploids (DH) is becoming an essential part of maize breeding programs worldwide as DH lines offer several advantages in line development and evaluation. One of the critical steps in maize DH line production is doubling the chromosomes of in vivo-derived haploids so that naturally sterile haploids become reproductively fertile diploids (DH) to produce seed. This step of artificially doubling the chromosomes is labor-intensive and costly; hence, optimizing protocols to improve the doubling success is critical for achieving efficiencies in the DH production pipelines. Immersion of 3–4-day old germinating haploid seedlings in colchicine solution is commonly used for chromosome doubling in large-scale maize DH line production. This manuscript presents a new method of colchicine application to haploid seedlings that showed superior doubling rates compared to other methods like standard seedling immersion, seed immersion, root immersion, and direct application of colchicine solution to the seedlings at V2 stage in the greenhouse trays. The new method involves immersing the crown region of the haploid seedlings along with all the seedling roots at V2 stage in the colchicine solution. Further experiments to optimize this method indicated that increasing colchicine concentration had a very positive effect on overall success rate in chromosomal doubling, while not drastically affecting survival rate. The optimized method showed on average 5.6 times higher overall success rate (OSR) compared to the standard haploid seedling immersion method which was the second-best method in our experiments. This improved method of colchicine application saves resources by reducing the number of haploids to be generated and handled in a maize DH production pipeline.
526			_aMCRP _bFP2 _bFP3
546			_aText in English
591			_aMartinez, L. : Not in IRS Staff list but CIMMYT Affiliation
591			_aOchieng, J.A.W. : Not in IRS Staff list but CIMMYT Affiliation
650		7	_aMaize _2AGROVOC _91173
650		7	_2AGROVOC _91925 _aHaploidy
650		7	_2AGROVOC _97014 _aChromosomes
650		7	_2AGROVOC _912122 _aColchicine
700	1		_aGowda, M. _9795 _8I1705963 _gGlobal Maize Program
700	1		_aMartinez, L. _93372
700	1		_aOchieng, J. _931316
700	0		_aHamilton Amoshe Omar _912123
700	1		_aPrasanna, B.M. _gGlobal Maize Program _8INT3057 _9887
773	0		_tPlants _gv. 9, no. 4, art. 459 _dBasel (Switzerland) : MDPI, 2020. _x2223-7747
856	4		_yOpen Access through DSpace _uhttps://hdl.handle.net/10883/20818
942			_cJA _n0 _2ddc
999			_c61625 _d61617