000 00595nab|a22002177a|4500
999 _c61613
_d61605
001 61613
003 MX-TxCIM
005 20200408193010.0
008 200325s2012||||xxu|||p|op||||00||0|eng|d
022 _a1943-3638 (Online)
024 8 _ahttps://doi.org/10.1094/CCHEM-07-11-0089
040 _aMX-TxCIM
041 _aeng
100 1 _aGamel, T.H.
_912062
245 1 0 _aApplication of the Rapid Visco Analyzer (RVA) as an effective rheological tool for measurement of β‐glucan viscosity
260 _aSt. Paul, MN (USA) :
_bAACC :
_bWiley,
_c2012.
500 _aPeer review
520 _aThe physiochemical characteristics of β‐glucan in oat and barley foods can affect human physiological response. A method for continuous measurement of β‐glucan viscosity with a Rapid Visco Analyzer (RVA) was developed to overcome the complexity of the common protocols based on in vitro digestion methods. The effects of several parameters on viscosity and solubility were considered. Oat cereal foods showed different RVA viscosity profiles depending on their physiochemical characteristics. Products high in starch exhibited a high initial viscosity that was reduced by α‐amylase action, whereas products with low amounts of starch exhibited a slow increase in viscosity. The viscosity of all samples reached a plateau in the viscosity curve after 1–2 hr, which is the key for obtaining reproducible results. Optimum digestion condition was achieved using sodium phosphate buffer (pH 6.9) and 1% β‐glucan dispersion at 37°C and 160 rpm. A particle size of <0.6 mm gave more consistent viscosities than did larger particles without affecting the solubility of β‐glucan. Pancreatin and α‐amylase concentrations affected the viscosity profile by influencing the digestion rate of protein and starch in the samples, but pepsin had limited influence at pH 6.9. Highly significant Pearson correlation between the in vitro digestibility protocol and RVA methods was achieved, indicting that the developed method could be used as an effective alternative for measurement of β‐glucan viscosity.
546 _aText in English
650 7 _2AGROVOC
_912063
_aBeta-glucans
650 7 _2AGROVOC
_912064
_aViscosity
650 7 _2AGROVOC
_99804
_aOats
650 7 _2AGROVOC
_91018
_aBarley
700 1 _912065
_aAbdel-Aal, E.M.
700 1 _9375
_aWood, P.J.
700 1 _912066
_aAmes, N.P.
700 1 _912067
_aTosh, S.M.
773 0 _tCereal Chemistry
_gv. 89, no. 1, p. 52-58
_dSt. Paul, MN (USA) : AACC : Wiley, 2012.
_x1943-3638
_wu444220
942 _cJA
_n0
_2ddc