000 | 02852nab a22003617a 4500 | ||
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999 |
_c61200 _d61192 |
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001 | 61200 | ||
003 | MX-TxCIM | ||
005 | 20220205062621.0 | ||
008 | 200108s2020 ne |||p|op||| 00| 0eng d | ||
022 | _a2214-5141 | ||
024 | 8 | _ahttps://doi.org/10.1016/j.cj.2019.09.008 | |
040 | _aMX-TxCIM | ||
041 | _aeng | ||
100 | 1 |
_8INT3232 _91434 _aNair, S.K. _gGlobal Maize Program |
|
245 | 1 | 0 | _aGenetic dissection of maternal influence on in vivo haploid induction in maize |
260 |
_aNetherlands : _bElsevier, _c2020. |
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500 | _aPeer review | ||
500 | _aOpen Access | ||
520 | _aIn vivo haploid induction based on maternal haploid inducers is the first step in deriving completely homozygous maize doubled haploid (DH) lines. Haploid induction rate (HIR) is influenced by both pollen parent inducing haploidy and the maternal source germplasm used in induction crosses. This study was aimed at analyzing the influence of source germplasm on HIR using 671 tropical inbred lines organized in two association mapping panels. These two association mapping panels (AMP1 and AMP2) were crossed to two different Tropically Adapted Inducer Lines (TAILs). For HIR assessment, seeds from induction crosses were planted in the field and ploidy status of each surviving plant was assessed using a gold standard classification based on visual differences between the haploid and diploid plants. The analysis revealed significant variation for HIR and led to identification of several tropical inbred lines that respond very positively to haploid induction. Use of HIR data in a genome wide association study (GWAS) led to identification of twenty-seven and two SNPs that were significantly associated with HIR in AMP1 and AMP2, respectively. Meta-analysis of AMP1 and AMP2 GWAS led to identification of 52 SNPs with significant effect on HIR across both studies. Genome-wide prediction revealed moderate to high prediction accuracy within AMPs using random SNPs. Inclusion of the SNPs detected in GWAS into the prediction model led to improvement in prediction accuracy. Overall, the study revealed that the maternal influence on HIR is controlled by a few moderate and many small effect QTL. | ||
526 |
_aMCRP _bFP2 _bFP3 |
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546 | _aText in English | ||
650 | 7 |
_aMaize _gAGROVOC _2 _91173 |
|
650 | 7 |
_2AGROVOC _91925 _aHaploidy |
|
650 | 7 |
_2AGROVOC _91132 _aGenomics |
|
700 | 1 |
_8INT3356 _9936 _aChaikam, V. _gGlobal Maize Program |
|
700 | 1 |
_8I1705963 _9795 _aGowda, M. _gGlobal Maize Program |
|
700 | 1 |
_96100 _aHindu, V. |
|
700 | 1 |
_93373 _aMelchinger, A.E. |
|
700 | 1 |
_aPrasanna, B.M. _gGlobal Maize Program _8INT3057 _9887 |
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773 | 0 |
_dNetherlands : Elsevier, 2020. _gv. 8, no. 2, p. 287-298 _tThe Crop Journal _wu56924 _x2214-5141 |
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856 | 4 |
_yOpen Access through DSpace _uhttps://hdl.handle.net/10883/20637 |
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942 |
_2ddc _cJA _n0 |