000			03142nab a22004697a 4500
999			_c60275 _d60267
001			60275
003			MX-TxCIM
005			20241108164544.0
008			190321s2016 ne ||||| |||| 00| 0 eng d
022			_a0167-4412
022			_a0167-4412 (Online)
040			_aMX-TxCIM
041			_aeng
100	1		_94403 _aPlett, D.
245	1	0	_aNitrogen assimilation system in maize is regulated by developmental and tissue-specific mechanisms
260			_aNetherlands : _bSpringer, _c2016.
500			_aPeer review
520			_aProgress in improving nitrogen (N) use efficiency (NUE) of crop plants has been hampered by the complexity of the N uptake and utilisation systems. To understand this complexity we measured the activities of seven enzymes and ten metabolites related to N metabolism in the leaf and root tissues of Gaspe Flint maize plants grown in 0.5 or 2.5 mM NO3 − throughout the lifecycle. The amino acids had remarkably similar profiles across the lifecycle except for transient responses, which only appeared in the leaves for aspartate or in the roots for asparagine, serine and glycine. The activities of the enzymes for N assimilation were also coordinated to a certain degree, most noticeably with a peak in root activity late in the lifecycle, but with wide variation in the activity levels over the course of development. We analysed the transcriptional data for gene sets encoding the measured enzymes and found that, unlike the enzyme activities, transcript levels of the corresponding genes did not exhibit the same coordination across the lifecycle and were only weakly correlated with the levels of various amino acids or individual enzyme activities. We identified gene sets which were correlated with the enzyme activity profiles, including seven genes located within previously known quantitative trait loci for enzyme activities and hypothesise that these genes are important for the regulation of enzyme activities. This work provides insights into the complexity of the N assimilation system throughout development and identifies candidate regulatory genes, which warrant further investigation in efforts to improve NUE in crop plants.
526			_aMCRP _bFP2
526			_aMCRP _bFP3
546			_aText in English
650		7	_2AGROVOC _96733 _aNitrogen metabolism
650		7	_2AGROVOC _94464 _aAmino acids
650		0	_aEnzyme activity _gAGROVOC _98773
650		7	_aMaize _gAGROVOC _2 _91173
700	1		_94404 _aHoltham, L.
700	1		_94405 _aBaumann, U.
700	1		_94406 _aKalashyan, E.
700	1		_94408 _aFrancis, K.L.
700	1		_94409 _aEnju, A.
700	1		_94410 _aToubia, J.
700	1		_94411 _aRoessner, U.
700	1		_94412 _aBacic, A.
700	1		_94413 _aRafalski, A.
700	1		_8I1706696 _aDhugga, K. _gGenetic Resource Program _94340
700	1		_94414 _aTester, M.
700	1		_94416 _aGarnett, T.
700	1		_94417 _aKaiser, B.N.
773	0		_gv. 92, no. 3, p. 293–312 _tPlant Molecular Biology _x0167-4412 _dNetherlands : Springer, 2016
856	4		_uhttp://libcatalog.cimmyt.org/Download/cis/60275.pdf _yAccess only for CIMMYT Staff
942			_2ddc _cJA _n0