000			nab a22 7a 4500
999			_c59993 _d59985
001			59993
003			MX-TxCIM
005			20191125161213.0
008			190122s2018 xxu|||po|p||| 00| 0 eng d
024	8		_ahttps://doi.org/10.1080/00275514.2018.1448167
040			_aMX-TxCIM
041			_aeng
100	1		_9419 _aAshrafi, S.
245	1		_aInhabiting plant roots, nematodes, and truffles—polyphilus, a new helotialean genus with two globally distributed species
260			_aUnited Kingdom : _bTaylor and Francis, _c2018.
500			_aPeer review
520			_aFungal root endophytes, including the common group of dark septate endophytes (DSEs), represent different taxonomic groups and potentially diverse life strategies. In this study, we investigated two unidentified helotialean lineages found previously in a study of DSE fungi of semiarid grasslands, from several other sites, and collected recently from a pezizalean truffle ascoma and eggs of the cereal cyst nematode Heterodera filipjevi. The taxonomic positions and phylogenetic relationships of 21 isolates with different hosts and geographic origins were studied in detail. Four loci, namely, nuc rDNA ITS1-5.8S-ITS2 (internal transcribed spacer [ITS]), partial 28S nuc rDNA (28S), partial 18S nuc rDNA (18S), and partial RNA polymerase II second-largest subunit (RPB2), were amplified and sequenced for molecular phylogenetic analyses. Analyses of similar ITS sequences from public databases revealed two globally distributed lineages detected in several biomes from different geographic regions. The host interaction of isolates from nematodes was examined using in vitro bioassays, which revealed that the fungi could penetrate nematode cysts and colonize eggs of H. filipjevi, confirming observations from field-collected samples. This is the first report of a DSE, and we are not aware of other helotialean fungal species colonizing the eggs of a plant-parasitic nematode. Neither conidiomata and conidia nor ascomata formation was detected in any of the isolates. Based on molecular phylogenetic analyses, these isolates represent a distinct lineage within the Helotiales in the Hyaloscyphaceae. For this lineage, we propose here the new genus Polyphilus represented by two new species, P. sieberi and P. frankenii.
526			_aWC _cFP3
546			_aText in English
650		7	_2AGROVOC _98313 _aGenus
650		7	_2AGROVOC _91284 _aTaxonomy
650		7	_2AGROVOC _91189 _aNew species
700	1		_98314 _aKnapp, D.G.
700	1		_98315 _aBlaudez, D.
700	1		_98316 _aChalot, M.
700	1		_98317 _aMacia-Vicente, J.G.
700	0		_98318 _aImre Zagyva
700	1		_9874 _aDababat, A.A. _gGlobal Wheat Program _8INT2918
700	1		_9190 _aMaier, W.
700	1		_98319 _aGabor M. Kovacs
773	0		_gv. 110, no. 2, p. 286-289 _tMycologia _x1557-2536 _wu444608
856	4		_uhttps://libcatalog.cimmyt.org/download/cis/59993.pdf _yAccess only for CIMMYT Staff
942			_2ddc _cJA _n0