000 03447nab a22005417a 4500
999 _c59420
_d59412
001 59420
003 MX-TxCIM
005 20211006075151.0
008 180416s2017 uik|||p|op||| 00| 0 eng d
024 8 _ahttps://doi.org/10.1186/s12915-017-0365-7
040 _aMX-TxCIM
041 _aeng
100 0 _96983
_aJianping Yu
245 1 0 _aOsLG3 contributing to rice grain length and yield was mined by Ho-LAMap
_h[Electronic Resource]
260 _aLondon, United Kingdom :
_bBioMed Central Ltd,
_c2017.
500 _aOpen Access
500 _aPeer review
520 _aBackground Most agronomic traits in rice are complex and polygenic. The identification of quantitative trait loci (QTL) for grain length is an important objective of rice genetic research and breeding programs. Results Herein, we identified 99 QTL for grain length by GWAS based on approximately 10 million single nucleotide polymorphisms from 504 cultivated rice accessions (Oryza sativa L.), 13 of which were validated by four linkage populations and 92 were new loci for grain length. We scanned the Ho (observed heterozygosity per locus) index of coupled-parents of crosses mapping the same QTL, based on linkage and association mapping, and identified two new genes for grain length. We named this approach as Ho-LAMap. A simulation study of six known genes showed that Ho-LAMap could mine genes rapidly across a wide range of experimental variables using deep-sequencing data. We used Ho-LAMap to clone a new gene, OsLG3, as a positive regulator of grain length, which could improve rice yield without influencing grain quality. Sequencing of the promoter region in 283 rice accessions from a wide geographic range identified four haplotypes that seem to be associated with grain length. Further analysis showed that OsLG3 alleles in the indica and japonica evolved independently from distinct ancestors and low nucleotide diversity of OsLG3 in indica indicated artificial selection. Phylogenetic analysis showed that OsLG3 might have much potential value for improvement of grain length in japonica breeding. Conclusions The results demonstrated that Ho-LAMap is a potential approach for gene discovery and OsLG3 is a promising gene to be utilized in genomic assisted breeding for rice cultivar improvement.
546 _aText in English
591 _aHuihui Li : No CIMMYT Affiliation
650 7 _aRice
_gAGROVOC
_2
_91243
650 7 _91853
_aQuantitative Trait Loci
_2AGROVOC
650 7 _96984
_aGenetic code
_2AGROVOC
650 7 _92084
_aChromosome mapping
_2AGROVOC
650 7 _98685
_aArtificial Selection
_2AGROVOC
700 0 _96985
_aHaiyan Xiong
700 0 _96986
_aXiaoyang Zhu
700 0 _96987
_aHongliang Zhang
700 0 _9764
_aHuihui Li
_gGenetic Resources Program
_8CLIH01
700 0 _96988
_aJinli Miao
700 0 _96989
_aWensheng Wang
700 0 _96990
_aZuoshun Tang
700 0 _96991
_aZhanying Zhang
700 0 _96992
_aGuoxin Yao
700 0 _96993
_aQiang Zhang
700 _96994
_aYinghua Pan
700 0 _96995
_aXin Wang
700 1 _96996
_aRashid, M.A.R.
700 0 _96997
_aJinjie Li
700 0 _96998
_aYongming Gao
700 0 _96999
_aZhikang Li
700 0 _97000
_aWeicai Yang
700 0 _97001
_aXiangdong Fu
700 0 _97002
_aZichao Li
773 0 _gv. 15, no. 28
_t BMC Biology
_x1741-7007
856 4 _yOpen Access through DSpace
_uhttps://hdl.handle.net/10883/19476
942 _2ddc
_cJA
_n0