000 | 03447nab a22005417a 4500 | ||
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999 |
_c59420 _d59412 |
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001 | 59420 | ||
003 | MX-TxCIM | ||
005 | 20211006075151.0 | ||
008 | 180416s2017 uik|||p|op||| 00| 0 eng d | ||
024 | 8 | _ahttps://doi.org/10.1186/s12915-017-0365-7 | |
040 | _aMX-TxCIM | ||
041 | _aeng | ||
100 | 0 |
_96983 _aJianping Yu |
|
245 | 1 | 0 |
_aOsLG3 contributing to rice grain length and yield was mined by Ho-LAMap _h[Electronic Resource] |
260 |
_aLondon, United Kingdom : _bBioMed Central Ltd, _c2017. |
||
500 | _aOpen Access | ||
500 | _aPeer review | ||
520 | _aBackground Most agronomic traits in rice are complex and polygenic. The identification of quantitative trait loci (QTL) for grain length is an important objective of rice genetic research and breeding programs. Results Herein, we identified 99 QTL for grain length by GWAS based on approximately 10 million single nucleotide polymorphisms from 504 cultivated rice accessions (Oryza sativa L.), 13 of which were validated by four linkage populations and 92 were new loci for grain length. We scanned the Ho (observed heterozygosity per locus) index of coupled-parents of crosses mapping the same QTL, based on linkage and association mapping, and identified two new genes for grain length. We named this approach as Ho-LAMap. A simulation study of six known genes showed that Ho-LAMap could mine genes rapidly across a wide range of experimental variables using deep-sequencing data. We used Ho-LAMap to clone a new gene, OsLG3, as a positive regulator of grain length, which could improve rice yield without influencing grain quality. Sequencing of the promoter region in 283 rice accessions from a wide geographic range identified four haplotypes that seem to be associated with grain length. Further analysis showed that OsLG3 alleles in the indica and japonica evolved independently from distinct ancestors and low nucleotide diversity of OsLG3 in indica indicated artificial selection. Phylogenetic analysis showed that OsLG3 might have much potential value for improvement of grain length in japonica breeding. Conclusions The results demonstrated that Ho-LAMap is a potential approach for gene discovery and OsLG3 is a promising gene to be utilized in genomic assisted breeding for rice cultivar improvement. | ||
546 | _aText in English | ||
591 | _aHuihui Li : No CIMMYT Affiliation | ||
650 | 7 |
_aRice _gAGROVOC _2 _91243 |
|
650 | 7 |
_91853 _aQuantitative Trait Loci _2AGROVOC |
|
650 | 7 |
_96984 _aGenetic code _2AGROVOC |
|
650 | 7 |
_92084 _aChromosome mapping _2AGROVOC |
|
650 | 7 |
_98685 _aArtificial Selection _2AGROVOC |
|
700 | 0 |
_96985 _aHaiyan Xiong |
|
700 | 0 |
_96986 _aXiaoyang Zhu |
|
700 | 0 |
_96987 _aHongliang Zhang |
|
700 | 0 |
_9764 _aHuihui Li _gGenetic Resources Program _8CLIH01 |
|
700 | 0 |
_96988 _aJinli Miao |
|
700 | 0 |
_96989 _aWensheng Wang |
|
700 | 0 |
_96990 _aZuoshun Tang |
|
700 | 0 |
_96991 _aZhanying Zhang |
|
700 | 0 |
_96992 _aGuoxin Yao |
|
700 | 0 |
_96993 _aQiang Zhang |
|
700 |
_96994 _aYinghua Pan |
||
700 | 0 |
_96995 _aXin Wang |
|
700 | 1 |
_96996 _aRashid, M.A.R. |
|
700 | 0 |
_96997 _aJinjie Li |
|
700 | 0 |
_96998 _aYongming Gao |
|
700 | 0 |
_96999 _aZhikang Li |
|
700 | 0 |
_97000 _aWeicai Yang |
|
700 | 0 |
_97001 _aXiangdong Fu |
|
700 | 0 |
_97002 _aZichao Li |
|
773 | 0 |
_gv. 15, no. 28 _t BMC Biology _x1741-7007 |
|
856 | 4 |
_yOpen Access through DSpace _uhttps://hdl.handle.net/10883/19476 |
|
942 |
_2ddc _cJA _n0 |