000			02850nab a22003257a 4500
999			_c59122 _d59114
001			59122
003			MX-TxCIM
005			20211006072241.0
008			180129s2018 ne |||p| p||| 00| 0 eng d
024	8		_ahttps://doi.org/10.1007/s10658-018-1420-0
040			_aMX-TxCIM
041			_aeng
100	1		_93200 _aMokrini, F.
245	1	0	_aInvestigation of resistance to Pratylenchus penetrans and P. thornei in international wheat lines and its durability when inoculated together with the cereal cyst nematode Heterodera avenae, using qPCR for nematode quantification
260			_aNetherlands : _bSpringer Nature, _c2018.
500			_aPeer review
520			_aThe root lesion nematodes Pratylenchus penetrans and P. thornei cause high yield losses in rain-fed wheat fields in Morocco, as well as worldwide. Growing resistant varieties is one of the most effective methods for controlling nematodes. Therefore, a collection of 14 lines of spring wheat and 11 lines of winter wheat (Triticum aestivum and T. durum), provided by CIMMYT, were screened for resistance to P. penetrans and P. thornei in tubes (15 × 20 × 120 mm3) under greenhouse conditions. The resistance level was evaluated based on the numbers of nematodes extracted from roots and soil 9 weeks after infestation. Three lines (L9, L12 and L13) were found moderately resistant (reproduction factor < 1) to P. thornei and one of these (L9) was also moderately resistant to P. penetrans. To investigate the durability of this resistance, we co-inoculated juveniles of Heterodera avenae, a cereal cyst nematode widely present in Moroccan wheat fields, and assessed the reproduction of both lesion nematodes on the three lines. Our results showed that the lines L9, L12, L13 remained moderately resistant in the presence of H. avenae. Moreover, the numbers of Pratylenchus spp. were generally lower when plants were co-inoculated with H. avenae. The number of P. penetrans or P. thornei were determined visually using a microscope and with a qPCR assay. The counts with qPCR were even lower than microscopic counts. These findings on resistance are promising but the field performance of these lines against root lesion nematode attacks should be evaluated.
546			_aText in English
650		7	_aWheat _gAGROVOC _2 _91310
650		7	_95194 _aInoculation _2AGROVOC
650		7	_91797 _aNematoda _gAGROVOC
650			_95621 _aScreening _2AGROVOC
700	1		_9358 _aViaene, N.
700	1		_9363 _aWaeyenberge, L.
700	1		_9874 _aDababat, A.A. _gGlobal Wheat Program _8INT2918
700	1		_9212 _aMoens, M.
773	0		_gv. 151, no. 4, p. 875-889 _tEuropean Journal of Plant Pathology _wu445058 _x(Print) 0929-1873 (Online) 1573-8469
856	4		_yAccess only for CIMMYT Staff _uhttps://libcatalog.cimmyt.org/Download/cis/59122.pdf
942			_2ddc _cJA _n0