000			03202nab a22003737a 4500
999			_c58962 _d58954
001			58962
003			MX-TxCIM
005			20211006085139.0
008			160128s2017 xxu|||p|op||| 00| 0 eng d
024	8		_ahttps://doi.org/10.1080/15427528.2017.1391915
040			_aCI01YT
041			_aeng
100	1		_95638 _aOkoth, S.
245	1	0	_aField evaluation of resistance to aflatoxin accumulation in maize inbred lines in Kenya and South Africa
260			_aNew York : _bTaylor and Francis, _c2017.
500			_aPeer review
520			_aAflatoxin, a carcinogenic toxin, is produced mainly by Aspergillus flavus and Aspergillus parasiticus. Contamination of maize (Zea mays L.) grain by these fungi occurs before harvest, and the easiest strategy to prevent this is to develop/use maize varieties resistant to Aspergillus spp. and aflatoxin accumulation. The objective of this investigation was to identify potential sources of resistance among 23 maize inbred lines (13 obtained from the MAIZE Competitive Grants Initiative, International Maize and Wheat Improvement Centre and 10 from Agricultural Research Council, South Africa). The inbred lines were planted in a randomized complete-block design at two locations each in Kenya and South Africa. Maize ears were inoculated at silking with three toxigenic strains of A. flavus. The inoculated ears in each plot were harvested at 12–18% moisture, dried, and visually assessed for Aspergillus ear rot (AER). Aflatoxin concentration in the kernels was determined using liquid chromatography–tandem mass spectrometry. Significant variation for both AER and aflatoxin concentration existed among the inbred lines at both locations in Kenya and one location in South Africa. Combined analysis revealed a significant (p < 0.001) lines × locations interaction for both AER and aflatoxin concentration. Higher incidences of AER (0–86.0%) and aflatoxin concentration (0.21–6.51 µg/kg) were recorded at Kiboko in Kenya than at the other three locations. A stronger genetic correlation (rG = 0.936, p < 0.0001) between the AER and aflatoxin concentration was recorded in Potchefstroom than at the other three locations. Repeatability of aflatoxin concentration was high at Kiboko (0.87) and Potchefstroom in South Africa (0.74). Three inbred lines, CML247, CML444, and CML495, emerged as potentially useful sources of resistance to AER and aflatoxin accumulation as they showed low levels of aflatoxin contamination in both localities in Kenya and in South Africa.
546			_aText in English
650		7	_aMaize _gAGROVOC _2 _91173
650		7	_95637 _aAflatoxins _2AGROVOC
651		7	_93783 _aKenya _2AGROVOC
651		7	_95594 _aSouth Africa _2AGROVOC
700	1		_95635 _aRose, L.J.
700	1		_95640 _aOuko, A.
700	1		_95639 _aBeukes, I.
700	1		_95785 _aSila, H.
700	1		_95641 _aMouton, M.
700	1		_95642 _aFlett, B.C.
700	1		_9858 _aMakumbi, D. _gGlobal Maize Program _8INT2765
700	1		_95643 _aViljoen, A.
773	0		_w94851 _tJournal of Crop Improvement _gv. 31, no. 6, p. 862-878
856	4		_uhttp://libcatalog.cimmyt.org/Download/cis/58962.pdf _yAccess only for CIMMYT Staff
942			_2ddc _cJA _n0