000			03538nab a22004097a 4500
999			_c58741 _d58733
001			58741
003			MX-TxCIM
005			20211006085136.0
008			151020s2017 sz |||p|op||| 00| 0 eng d
024	8		_ahttps://doi.org/10.3389/fpls.2017.01022
040			_aCI10YT
041			_aeng
100	0		_95051 _aHewei Du
245	1	0	_aBulked segregant RNA-seq reveals differential expression and SNPs of candidate genes associated with waterlogging tolerance in maize _h[Electronic Resource]
260			_aSwitzerland : _bFrontiers, _c2017.
500			_aPeer review
500			_aOpen Access
520			_aWaterlogging has increasingly become one of the major constraints to maize productivity in some maize production zones because it causes serious yield loss. Bulked segregant RNA-seq (BSR-seq) has been widely applied to profile candidate genes and map associated Single Nucleotide Polymorphism (SNP) markers in many species. In this study, 10 waterlogging sensitive and eight tolerant inbred lines were selected from 60 maize inbred lines with waterlogging response determined and preselected by the International Maize and Wheat Improvement Center (CIMMYT) from over 400 tropical maize inbred lines. BSR-seq was performed to identify differentially expressed genes and SNPs associated with waterlogging tolerance. Upon waterlogging stress, 354 and 1094 genes were differentially expressed in the tolerant and sensitive pools, respectively, compared to untreated controls. When tolerant and sensitive pools were compared, 593 genes were differentially expressed under untreated and 431 genes under waterlogged conditions, of which 122 genes overlapped. To validate the BSR-seq results, the expression levels of six genes were determined by qRT-PCR. The qRT-PCR results were consistent with BSR-seq results. Comparison of allelic polymorphism in mRNA sequences between tolerant and sensitive pools revealed 165 (normal condition) and 128 (waterlogged condition) high-probability SNPs. We found 18 overlapping SNPs with genomic positions mapped. Eighteen SNPs were contained in 18 genes, and eight and nine of 18 genes were responsive to waterlogging stress in tolerant and sensitive lines, respectively. Six alleles of the 18 originated from tolerant pool were significantly up-regulated under waterlogging, but not those from sensitive pool. Importantly, one allele (GRMZM2G055704) of the six genes was mapped between umc1619 and umc1948 on chromosome 1 where a QTL associated with waterlogging tolerance was identified in a previous research, strongly indicating that GRMZM2G055704 is a candidate gene responsive to waterlogging. Our research contributes to the knowledge of the molecular mechanism for waterlogging tolerance in maize.
546			_aText in English
650		7	_99041 _aRNA Sequence _2AGROVOC
650		7	_95053 _aWater tolerance _2AGROVOC
650		7	_aMaize _gAGROVOC _2 _91173
650		7	_91937 _aNucleotide sequence _2AGROVOC
700	0		_95054 _aJianxiong Zhu
700	0		_95055 _aHang Su
700	0		_95056 _aMing Huang
700	0		_95057 _aHongwei Wang
700	0		_95058 _aShuangcheng Ding
700	0		_95059 _aBinglin Zhang
700	0		_95060 _aAn Luo
700	0		_95061 _aShudong Wei
700	0		_95062 _aXiaohai Tian
700	1		_9857 _aYunbi Xu _gGlobal Maize Program _8INT2735
773	0		_wu56875 _x1664-462X _dSwitzerland : Frontiers _tFrontiers in Plant Science _gv. 8, no. 1022, p. 1-13
856	4		_uhttps://hdl.handle.net/10883/18874 _yOpen Access through DSpace
942			_2ddc _cJA _n0