000			03215nab a22003377a 4500
999			_c58548 _d58540
001			58548
003			MX-TxCIM
005			20211006082126.0
008			150723s2017 xxu|||po p||| 00| 0 eng d
024	8		_ahttps://doi.org/10.1371/journal.pone.0171963
040			_aMX-TxCIM
041			_aeng
100	0		_94696 _aLong-Xi Yu
245	1	0	_aIdentification and validation of single nucleotide polymorphic markers linked to Ug99 stem rust resistance in spring wheat _h[Electronic Resource]
260			_aSan Francisco, USA : _bPublic Library of Science, _c2017.
500			_aOpen Access
500			_aPeer review
520			_aWheat stem rust (Puccinia graminis f. sp. tritici Eriks. and E. Henn.) is one of the most destructive diseases world-wide. Races belonging to Ug99 (or TTKSK) continue to cause crop losses in East Africa and threaten global wheat production. Developing and deploying wheat varieties with multiple race-specific genes or complex adult plant resistance is necessary to achieve durability. In the present study, we applied genome-wide association studies (GWAS) for identifying loci associated with the Ug99 stem rust resistance (SR) in a panel of wheat lines developed at the International Maize and Wheat Improvement Center (CIMMYT). Genotyping was carried out using the wheat 9K iSelect single nucleotide polymorphism (SNP) chip. Phenotyping was done in the field in Kenya by infection of Puccinia graminis f. sp. tritici race TTKST, the Sr24-virulent variant of Ug99. Marker-trait association identified 12 SNP markers significantly associated with resistance. Among them, 7 were mapped on five chromosomes. Markers located on chromosomes 4A and 4B overlapped with the location of the Ug99 resistance genes SrND643 and Sr37, respectively. Markers identified on 7DL were collocated with Sr25. Additional significant markers were located in the regions where no Sr gene has been reported. The chromosome location for five of the SNP markers was unknown. A BLASTN search of the NCBI database using the flanking sequences of the SNPs associated with Ug99 resistance revealed that several markers were linked to plant disease resistance analogues, while others were linked to regulatory factors or metabolic enzymes. A KASP (Kompetitive Allele Specific PCR) assay was used for validating six marker loci linked to genes with resistance to Ug99. Of those, four co-segregated with the Sr25-pathotypes while the rest identified unknown resistance genes. With further investigation, these markers can be used for marker-assisted selection in breeding for Ug99 stem rust resistance in wheat.
546			_aText in English
650		7	_94697 _aNucleotides _2AGROVOC
650		7	_aRusts _gAGROVOC _2 _91251
650		7	_91806 _aSpring wheat _2AGROVOC
650		7	_92084 _aChromosome mapping _2AGROVOC
650		7	_91848 _aGenetic markers _2AGROVOC
700	1		_93777 _aShiaoman Chao
700	1		_aSingh, R.P. _gGlobal Wheat Program _8INT0610 _9825
700	1		_92094 _aSorrells, M.E.
773	0		_wu94957 _dSan Francisco, CA (USA) : Public Library of Science _tPLoS One _gv. 12, no. 2 : e0171963
856	4		_yOpen Access through DSpace _uhttps://hdl.handle.net/10883/18608
942			_2ddc _cJA _n0