000			02326nab a22003377a 4500
999			_c58282 _d58274
001			58282
003			MX-TxCIM
005			20211006085132.0
008			150721s2015 xxu|||p|op||| 00| 0 eng d
024	8		_ahttps://doi.org/10.1111/jipb.12467
040			_aMX-TxCIM
041			_aeng
100	0		_94233 _aDongdong Xu
245	1	0	_aIdentification and functional characterization of the AGO1 ortholog in maize _h[Electronic Resource]
260			_aOxford : _bWiley-Blackwell, _c2016.
500			_aPeer review
500			_aOpen Access
520			_aEukaryotic Argonaute proteins play primary roles in miRNA and siRNA pathways that are essential for numerous developmental and biological processes. However, the functional roles of the four ZmAGO1 genes have not yet been characterized in maize (Zea mays L.). In the present study, ZmAGO1a was identified from four putative ZmAGO1 genes for further characterization. Complementation of the Arabidopsis ago1-27 mutant with ZmAGO1a indicated that constitutive overexpression of ZmAGO1a could restore the smaller rosette, serrated leaves, later flowering and maturation, lower seed set, and darker green leaves at late stages of the mutant to the wild-type phenotype. The expression profiles of ZmAGO1a under five different abiotic stresses indicated that ZmAGO1a shares expression patterns similar to those of Argonaute genes in rice, Arabidopsis, and wheat. Further, variation in ZmAGO1a alleles among diverse maize germplasm that resulted in several amino acid changes revealed genetic diversity at this locus. The present data suggest that ZmAGO1a might be an important AGO1 ortholog in maize. The results presented provide further insight into the function of ZmAGO1a.
526			_aMCRP _bFP2 _bFP3
650		7	_aMaize _gAGROVOC _2 _91173
650		7	_99041 _aRNA Sequence _2AGROVOC
650		7	_98831 _aGenetic engineering _gAGROVOC
700	0		_94234 _aHailong Yang
700	0		_94196 _aCheng Zou
700	0		_92733 _aWen-Xue Li
700	1		_9857 _aYunbi Xu _gGlobal Maize Program _8INT2735
700	0		_92730 _aChuanxiao Xie
773	0		_wu56920 _x1744-7909 (Online) _dOxford : Wiley-Blackwell, 2005- _tJournal of integrative plant biology _gv. 58, no. 8, p. 749-758
856	4		_uhttp://hdl.handle.net/10883/18837 _yOpen Access through DSpace
942			_2ddc _cJA _n0