000			02805nab a22003737a 4500
999			_c57311 _d57303
001			57311
003			MX-TxCIM
005			20211006085127.0
008			160125s2015 ne |||p|op||| 00| 0 eng d
024	8		_ahttps://doi.org/10.1007/s11032-015-0349-7
040			_aMX-TxCIM
041			_aeng
100	1		_9573 _aLiu, L.
245	1	0	_aIntrogression of the crtRB1 gene into quality protein maize inbred lines using molecular markers
260			_aDordrecht (Netherlands) : _bSpringer, _c2015.
500			_aPeer review
500			_aOpen Access
520			_aQuality protein maize (QPM; Zea mays L.) has effectively enhanced levels of the amino acids, lysine, and tryptophan, over normal maize and provided balanced dietary protein for the health and development of monogastric animals and humans. However, as in normal maize, QPM varieties are low in provitamin A (ProVA), a precursor of vitamin A, which can lead to vitamin A deficiency in humans when maize is a significant part of their diet. In this study, maize inbred Hp321-1 carrying the favorable alleles crtRB1-50 TE-2 and crtRB1-30 TE-1 that can enhance levels of ProVA, was used as donor for improving ProVA in QPM inbred lines CML161 and CML171. Functional markers for identifying the favorable alleles crtRB1-50 TE-2 and crtRB1-30 TE-1 were used in foreground selection, and simple sequence repeat markers were used in background selection for the BC1F1, BC2F1, and BC2F2 generations. The background recovery rates were 77.4 and 84.5 % for CML161 and CML171 populations, respectively, in the BC1F1 generation, and 89.9 and 92.1 % in the BC2F2 generation. With foreground and background selection, the mean ProVA concentration has been improved from 1.60 lg g-1 in the parent of CML161 to 5.25 lg g-1 in its BC2F3 offspring, from 1.80 lg g-1 in the parent of CML171 to 8.14 lg g-1 in its BC2F3 offspring while maintaining similar QPM characteristics of the recurrent parents. The success from this study offers maize breeders a procedure for increasing ProVA in QPM lines, which will greatly mitigate vitamin A defi- ciency and protein-energy malnutrition in developing countries.
536			_aGlobal Maize Program
546			_aText in english
594			_aINT1482
650		7	_91241 _aRetinol _2AGROVOC
650		7	_aInbred lines _gAGROVOC _2 _91155
650		7	_aMaize _gAGROVOC _2 _91173
700	1		_91465 _aZhang Yudong
700	0		_92612 _aMeiling Ding
700	0		_92613 _aWei Chen
700	1		_92614 _aKang, M.S.
700	1		_91466 _aFan Xingming
700	1		_9830 _aJeffers, D.P. _gGlobal Maize Program _8INT1482
773	0		_wu78961 _x1380-3743 _dDordrecht (Netherlands) : Springer _tMolecular Breeding _gv. 35, p. 154
856	4		_yOpen Access through DSpace _uhttps://hdl.handle.net/10883/19751
942			_2ddc _cJA _n0