000			03292nab a22003857a 4500
999			_c57239 _d57231
001			57239
003			MX-TxCIM
005			20200819163558.0
008			160106s2015 xxu|||p|op||| 00| 0 eng d
022			_a1932-6203 (Online)
024	8		_ahttps://doi.org/10.1371/journal.pone.0122165
040			_aMX-TxCIM
041			_aeng
100	1		_9922 _aSehgal, D. _gGlobal Wheat Program _8INT3332
245	1	0	_aExploring potential of pearl millet germplasm association panel for association mapping of drought tolerance traits
260			_aSan Francisco, CA (USA) : _bPublic Library of Science, _c2015.
500			_aPeer review
500			_aOpen Access
520			_aA pearl millet inbred germplasm association panel (PMiGAP) comprising 250 inbred lines, representative of cultivated germplasm from Africa and Asia, elite improved open-pollinated cultivars, hybrid parental inbreds and inbred mapping population parents, was recently established. This study presents the first report of genetic diversity in PMiGAP and its exploitation for association mapping of drought tolerance traits. For diversity and genetic structure analysis, PMiGAP was genotyped with 37 SSR and CISP markers representing all seven linkage groups. For association analysis, it was phenotyped for yield and yield components and morpho-physiological traits under both well-watered and drought conditions, and genotyped with SNPs and InDels from seventeen genes underlying a major validated drought tolerance (DT) QTL. The average gene diversity in PMiGAP was 0.54. The STRUCTURE analysis revealed six subpopulations within PMiGAP. Significant associations were obtained for 22 SNPs and 3 InDels from 13 genes under different treatments. Seven SNPs associations from 5 genes were common under irrigated and one of the drought stress treatments. Most significantly, an important SNP in putative acetyl CoA carboxylase gene showed constitutive association with grain yield, grain harvest index and panicle yield under all treatments. An InDel in putative chlorophyll a/b binding protein gene was significantly associated with both stay-green and grain yield traits under drought stress. This can be used as a functional marker for selecting high yielding genotypes with ‘stay green’ phenotype under drought stress. The present study identified useful marker-trait associations of important agronomics traits under irrigated and drought stress conditions with genes underlying a major validated DT-QTL in pearl millet. Results suggest that PMiGAP is a useful panel for association mapping. Expression patterns of genes also shed light on some physiological mechanisms underlying pearl millet drought tolerance.
536			_aGlobal Wheat Program
546			_aText in English
594			_aINT3332
650		7	_92433 _aPennisetum glaucum _2AGROVOC
650		7	_91082 _aDrought tolerance _2AGROVOC
650		7	_98811 _aMillets _2AGROVOC
650		7	_96794 _aCereal crops _2AGROVOC
700	1		_92434 _aSkot, L.
700	1		_92435 _aSingh, R.
700	0		_92436 _aRakesh Kumar Srivastava
700	0		_92437 _aSankar Prasad Das
700	1		_92438 _aTaunk, J.
700	1		_92439 _aSharma, P.C.
700	1		_92440 _aPal, R.
700	1		_92441 _aRaj, B.
700	1		_92442 _aHash, C.T.
700	1		_92443 _aYadav, R.S.
773	0		_wu94957 _dSan Francisco, CA (USA) : Public Library of Science, 2015. _tPLoS One _gv. 10, no. 5, p. 1-28
856	4		_yOpen Access through DSpace _uhttps://hdl.handle.net/10883/19708
942			_2ddc _cJA _n0