000			02743nab a22004217a 4500
999			_c57149 _d57141
001			57149
003			MX-TxCIM
005			20240919021225.0
008			151130s2015 n |||p|op||| 00| 0 eng d
024	8		_ahttps://doi.org/10.1016/j.cpb.2015.05.001
040			_aMX-TxCIM
041			_aeng
100	0		_92189 _aPunam S. Yadav
245	1	0	_aEnhanced resistance in wheat against stem rust achieved by marker assisted backcrossing involving three independent Sr genes
260			_aAmsterdam : _bElsevier, _c2015.
500			_aOpen Access
500			_aPeer review
520			_aMarker assisted backcrossing (MABC) was used to transfer the three stem rust (Puccinia graminis tritici)resistant genes Sr25, SrWeb and Sr50 into the popular Indian wheat cv. HUW234. The donor was theCIMMYT breeding line PMBWIR4, and each of the target genes was marked by a simple PCR assay. Athree step screening strategy was adopted: the first screen comprised foreground selection, the secondused markers lying close to the introgression borders to reduce the extent of linkage drag, and the last wasbackground selection based on 149 informative microsatellite assays. Based on the background selectionoutcomes, at the BC1F1generation the proportion of the genome fixed for cv. HUW234 alleles rangedfrom 55% to 67%. Three BC1F1individuals were crossed a second time with cv. HUW234, resulting inthe identification of six BC2F3families carrying all three target Sr genes; the proportion of their genomeinherited from cv. HUW234 ranged from 86% to 92%. BC2F4and BC2F5material was planted at threedifferent locations under both disease-free and artificially induced epiphytotic conditions. Compared torecurrent parent, the improved versions of cv. HUW234 displayed significantly superior resistance tostem rust, and their agronomic performance was as good as, or even marginally better than that of thesource cultivar.
536			_aGlobal Wheat Program
546			_aText in english
591			_bCIMMYT Informa No. 1960
594			_aINT2917
650		7	_aRusts _gAGROVOC _2 _91251
650		7	_aPlant breeding _gAGROVOC _2 _91203
650		7	_aDisease resistance _gAGROVOC _2 _91077
650		7	_93444 _aMolecular genetics _2AGROVOC
700	0		_91734 _aVinod Kumar Mishra
700	1		_91876 _aArun, B.
700	0		_aRamesh Chand _91735
700	1		_92190 _aVishwakarma, M.K.
700	1		_92191 _aVasistha, N.K.
700	1		_92192 _aMishra, A.N.
700	1		_91874 _aKalappanavar, I.K.
700	1		_9873 _aJoshi, A.K. _gGlobal Wheat Program _8INT2917
773	0		_w57148 _x2214-6628 _dAmsterdam : Elsevier _tCurrent plant biology _gv. 2, p. 25-33
856	4		_yOpen Access through DSpace _uhttp://hdl.handle.net/10883/16817
942			_2ddc _cJA _n0