000 02736nam a22004457a 4500
001 G68374
003 MX-TxCIM
005 20211006075506.0
008 121211s ||||f| 0 p|p||0|| |
020 _a970-648-035-8
040 _aMX-TxCIM
072 0 _aF30
072 0 _aH20
082 0 4 _a632.4
_bGIN
100 1 _aMurphy, N.E.A.
_uSeptoria and Stagonospora Diseases of Cereals: A Compilation of Global Research. Proceedings of International Workshop, 5. Mexico, D.F. (Mexico); 20-24 Sep 1999
110 2 _aCentro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT), Mexico DF (Mexico)
245 0 0 _aIdentification of a molecular marker linked to Septoria nodorum blotch resistance in Triticum tauschii using F2 bulked segregant
260 _aMexico, DF (Mexico)
_bCIMMYT :
_c1999
340 _aPrinted
520 _aA search was conducted for a molecular marker linked to a gene for resistance to septoria nodorum blotch in the Triticum tauschii accession RL5271. DNA was extracted from leaves of F2 plants which were progeny tested to identify homozygous resistant and homozygous susceptible F2 plants. The DNA from the homozygous resistant plants was pooled together and the low copy sequences were enriched using renaturation kinetics and hydroxyapatite to remove the repetitive DNA sequences. The pooled DNA from the homozygous susceptible plants was treated in the same manner. The pooled DNA and the parental DNA were screened using RAPD primers. Two markers present in the pooled resistant DNA and in the parental DNA were identified and cloned. These markers were verified using RFLPs with the cloned marker as a probe. One of the probes did not produce any polymorphism as a RFLP, even when a number of different restriction enzymes were used. The second marker was polymorphic between the two parents when the DNA was restricted with HindIII and then MseI. In the 14 homozygous F2 plants tested, the marker was completely linked to the resistance gene. This marker may be valuable in introgressing the resistance gene from T. tauschii into a commercial bread wheat cultivar.
546 _aEnglish
591 _a9910|AGRIS 0001
593 _aJose Juan Caballero
595 _aCPC
650 1 0 _aDNA
650 1 0 _aGenetic resistance
_92277
650 1 0 _aIdentification
650 1 0 _aMolecular genetics
650 1 7 _aPlant diseases
_gAGROVOC
_2
_91206
650 1 0 _aSeptoria
653 0 _aCIMMYT
650 1 7 _aTriticum
_gAGROVOC
_2
_91295
700 1 _91980
_aAppels, R.
700 1 _aGinkel, M. van|McNab, A.|Krupinsky, J.
_eeds.
700 1 _aJones, M.G.K.,
_ecoaut.
700 1 _aLagudah, E.S.,
_ecoaut.
_91697
700 1 _aLoughman, R.,
_ecoaut.
700 1 _aWilson, R.E.,
_ecoaut.
942 _cPRO
999 _c4389
_d4389