000			02840nam a22004817a 4500
001			G68360
003			MX-TxCIM
005			20211006075506.0
008			121211s ||||f| 0 p|p||0|| |
020			_a970-648-035-8
040			_aMX-TxCIM
072		0	_aH20
082	0	4	_a632.4 _bGIN
100	1		_aHamza, S. _uSeptoria and Stagonospora Diseases of Cereals: A Compilation of Global Research. Proceedings of International Workshop, 5. Mexico, D.F. (Mexico); 20-24 Sep 1999
110	2		_aCentro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT), Mexico DF (Mexico)
245	0	0	_aCharacterization of Septoria tritici variants and PCR assay for detecting Stagonospora nodorum and Septoria tritici in wheat
260			_aMexico, DF (Mexico) _bCIMMYT : _c1999
340			_aPrinted
520			_aPathogenic specialization of Septoria tritici was studied by inoculating 14 isolates of the fungus, of which seven were obtained from durum wheat and seven were isolated from bread wheat. Isolates obtained from durum wheat were more virulent on durum wheat, while those isolated from bread wheat were more severe on bread wheat, which revealed physiologic specialization of S. tritici to either bread or durum wheat. The sequence coding for the nuclear 5.8S rDNA and the internal transcribed spacer (ITS1 and ITS2) were amplified by polymerase chain reaction and sequenced for five isolates adapted to bread wheat and five isolates adapted to durum wheat. These sequences were identical between both variants, resulting in the absence of divergence inside tritici species. Septoria tritici and Stagonospora nodorum isolates collected from Tunisia were tested for amplification with specific primers to these pathogens. This revealed the primer’s efficiency to distinguish Septoria species and detect Stagonospora nodorum DNA with as little as 2 pg of DNA. Time course quantification of S. tritici mycelia after inoculation of resistant and susceptible cultivars distinguished those cultivars by the earliest date of apparition of a PCR product.
546			_aEnglish
591			_a9910|AGRIS 0001
593			_aJose Juan Caballero
595			_aCPC
650	1	7	_aDisease resistance _gAGROVOC _2 _91077
650	1	0	_aDNA
650	1	0	_aPathogens _91197
650	1	7	_aPlant diseases _gAGROVOC _2 _91206
650	1	0	_aSeptoria
650	1	0	_aStagonospora
653	0		_aCIMMYT
650	1	7	_aTriticum _gAGROVOC _2 _91295
650	1	7	_aWheat _gAGROVOC _2 _91310
700	1		_aAbdennour, S., _ecoaut.
700	1		_aCherif, M., _ecoaut.
700	1		_aGinkel, M. van|McNab, A.|Krupinsky, J. _eeds.
700	1		_aHarrabi, M., _ecoaut.
700	1		_aMedini, M., _ecoaut.
700	1		_aRouassi, M., _ecoaut.
700	1		_aSalah, A.B., _ecoaut.
700	1		_aSassi, T., _ecoaut.
700	1		_aStrange, R., _ecoaut.
942			_cPRO
999			_c4375 _d4375