000			03010nam a22005057a 4500
001			G66386
003			MX-TxCIM
005			20240919021051.0
008			121211s ||||f| 0 p|p||0|| |
020			_a968-6923-94-2
040			_aMX-TxCIM
072		0	_aF30
072		0	_aH20
082	0	4	_a632.4 _bDUB
100	1		_aNicholson, P. _uFusarium Head Scab: Global Status and Future Prospects; Proceedings of a Workshop; El Batan, Mexico; 13-17 Oct 1996
110	2		_aCentro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT), Mexico DF (Mexico)
245	0	0	_aDetection and quantification of individual fungal species in fusarium ear blight by PCR
260			_aMexico, DF (Mexico) _bCIMMYT : _c1997
340			_aPrinted
520			_aUntil recently, no method was available to identify and quantify individual Fusarium species present within plant tissue. Specific DNA markers have been identified for the Fusarium species that are predominant components of the 'fusarium ear blight complex' (scab) of cereals. In the UK and continental Europe, two varieties of Microdochium nivale (var. majus and var. nivale),formerly F. nivale, are also involved in the ear blight disease complex. Assays based on the polymerase chain reaction (PCR) have been developed for detection of several of these fungi in DNA extracts from plant tissue. These assays have been refined to enable quantification of each species, allowing the relative contribution of each component to the disease of the plant to be estimated. The role of trichothecene mycotoxins in the pathogenicity of Fusarium species towards cereals is also being investigated. Attempts to disrupt the Tri5 gene of F. culmorum are described, along with the results of experiments using Tri5 disruptants of F. graminearum. Visual disease scoring and quantitative PCR are being used to investigate the effects of Tri5 gene disruption on the infection of seedlings and ears of wheat. Examples of the use of PCR techniques in epidemiological studies involving F. poae, F. culmorum, F. graminearum, F. avenaceum, and M. nivale varieties are also presented. Other work ongoing in the research program is summarized.
546			_aEnglish
591			_a9806|AGRIS 9802
593			_aJose Juan Caballero
595			_aCPC
650	1	0	_aBlights
650	1	7	_aBreeding methods _gAGROVOC _2 _91030
650	1	0	_aDisease control
650	1	0	_aEpidemiology
650	1	7	_aFungi _gAGROVOC _2 _91121
650	1	0	_aFusarium _92705
650	1	0	_aPathogens _91197
650	1	7	_aPlant diseases _gAGROVOC _2 _91206
653	0		_aCIMMYT
650	1	7	_aTriticum _gAGROVOC _2 _91295
650	1	7	_aPlant breeding _gAGROVOC _2 _91203
700	1		_aDoohan, F., _ecoaut.
700	1		_aDubin, H.J.|Gilchrist, L.|Reeves, J.|McNab, A. _eeds.
700	1		_aJoyce, D., _ecoaut.
700	1		_aRezanoor, H.N., _ecoaut.
700	1		_aSimpson, D., _ecoaut.
700	1		_aSmith, P.H., _ecoaut.
700	1		_aTurner, A., _ecoaut.
700	1		_aWeston, G., _ecoaut.
942			_cPRO
999			_c3872 _d3872