000			02109nab a22003137a 4500
001			G99611
003			MX-TxCIM
005			20210505163929.0
008			121211b |||p||p||||||| |z||| |
022	0		_a0031-9317
024	8		_ahttps://doi.org/10.1111/ppl.12294
040			_aMX-TxCIM
041	0		_aEn
100	1		_aAranjuelo, I.
245	0	0	_aA novel method for determination of the 15N isotopic composition of Rubisco in wheat plants exposed to elevated atmospheric carbon dioxide
260			_c2015
500			_aPeer-review: Yes - Yes |http://ip-science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&Full=Physiologia%20Plantarum
520			_aAlthough ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is mostly known as a key enzyme involved in CO2 assimilation during the Calvin cycle, comparatively little is known about its role as a pool of nitrogen storage in leaves. For this purpose, we developed a protocol to purify Rubisco that enables later analysis of its 15N isotope composition (δ15N) at the natural abundance and 15N-labeled plants. In order to test the utility of this protocol, durum wheat (Triticum durum var. Sula) exposed to an elevated CO2 concentration (700 vs 400 ?mol mol−1) was labeled with K15NO3 (enriched at 2 atom %) during the ear development period. The developed protocol proves to be selective, simple, cost effective and reproducible. The study reveals that 15N labeling was different in total organic matter, total soluble protein and the Rubisco fraction. The obtained data suggest that photosynthetic acclimation in wheat is caused by Rubisco depletion. This depletion may be linked to preferential nitrogen remobilization from Rubisco toward grain filling.
536			_aGlobal Wheat Program
546			_aEnglish
591			_aWiley|CIMMYT Informa 2015
594			_aINT3193
595			_aCSC
700	1		_aAvice, J.C., _ecoaut.
700	1		_aBourguignon, J., _ecoaut.
700	1		_9899 _aMolero, G. _gFormerly Global Wheat Program _8INT3193
773	0		_tPhysiologia Plantarum _gv. 153, no. 2, p. 195-203
942			_cJA _2ddc
999			_c30903 _d30903