000			02872nab a22003137a 4500
001			G99509
003			MX-TxCIM
005			20220205062612.0
008			121211b |||p||p||||||| |z||| |
024	8		_ahttps://doi.org/10.1007/s00122-014-2419-3
040			_aMX-TxCIM
041	0		_aeng
100	1		_9936 _aChaikam, V. _gGlobal Maize Program _8INT3356
245	0	0	_aAnalysis of effectiveness of R1-nj anthocyanin marker for in vivo haploid identification in maize and molecular markers for predicting the inhibition of R1-nj expression
260			_c2015. _aUSA: _bSpringer,
500			_aPeer-review: Yes - Yes | http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0040-5752
520			_aThe R1-Navajo (R1-nj) color marker facilitates easy and quick identification of haploid kernels at the seed stage during in vivo haploid induction process in maize. However, the Navajo phenotype can be completely suppressed or poorly expressed in some germplasm, making it impossible or inefficient to identify haploids at the seed stage. In this study, we characterized the expression of R1-nj marker in a large array of tropical/subtropical inbred lines, breeding populations and landraces by crossing with the R1-nj-based tropicalized haploid inducer. There was a high frequency of inhibition of the Navajo phenotype in the maize inbred lines, which are used in tropical breeding programs. Genome-wide association mapping showed that the C1 anthocyanin regulatory locus is the most significant genetic factor influencing inhibition of the Navajo phenotype. Molecular marker assays were designed based on polymorphism in the C1 vs C1-I alleles. Analysis of a set of 714 inbred lines demonstrated that a combination of two gene-specific markers?8 bp C1-I InDel and C1-I SNP?could predict with high accuracy the presence of anthocyanin color inhibition in the germplasm analyzed. Information generated in this study aids in making informed decisions on the constitution of source populations for doubled haploid (DH) line development in tropical germplasm, particularly those derived from elite maize lines from CIMMYT. The C1-I gene-specific molecular markers identified and validated will facilitate high-throughput and cost-effective evaluation of a large pool of germplasm for the presence of the dominant color inhibitor in maize germplasm.
536			_aGlobal Maize Program
546			_aEnglish
591			_aCIMMYT Informa No. 1914
594			_aINT3356|INT3232|INT2925|CTEJ01|INT3057
700	1		_aMartinez, L., _ecoaut.
700	1		_aTejomurtula, J., _ecoaut.
700	1		_9875 _aBABU, R. _gGlobal Maize Program _8INT2925
700	1		_aPrasanna, B.M. _gGlobal Maize Program _8INT3057 _9887
700	1		_91434 _aNair, S.K. _gGlobal Maize Program _8INT3232
773	0		_tTheoretical and Applied Genetics _gv. 128, p. 159-171
942			_cJA _2ddc
999			_c30863 _d30863