000			03033nab a22004577a 4500
001			G98112
003			MX-TxCIM
005			20230417202533.0
008			210709s2013 ne |||p|op||| 00| 0 eng d
022			_a1568-5411 (Online)
022	0		_a1388-5545
024	8		_ahttps://doi.org/10.1163/15685411-00002713
040			_aMX-TxCIM
041			_aeng
090			_aCIS-7261
100	1		_9404 _aToumi, F.
245	1	0	_aDevelopment of a species-specific PCR to detect the cereal cyst nematode, Heterodera latipons
260			_aLeiden (Netherlands) : _bBrill, _c2013.
500			_aPeer review
500			_aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=1388-5545
520			_aSeveral Heterodera species can reduce the yield of wheat and barley, among which H. avenae, H. filipjevi and H. latipons are economically the most important. Their identification, based on morphological characteristics, is not straightforward but can be made easier using molecular techniques. In this study, we developed species-specific primers for the detection of H. latipons. The actin gene of eight Heterodera species was partially sequenced and, after purifying and sequencing the PCR products, all sequences were aligned to find unique sites. The alignment showed moderate to very high similarities between the species. However, a small fragment of the actin gene was suitable for the construction of a potentially useful species-specific primer for H. latipons. The optimised PCR was subsequently tested with several populations of 14 Heterodera species and a single population of Punctodera punctata. Heterodera latipons was represented by 16 populations originating from six different countries. The primer set (Hlat-act), designed using AlleleID 7.73, was shown to be very specific. To test its sensitivity further, the PCR was conducted on DNA extracted from five second-stage juveniles (J2) of H. latipons mixed with five or 100 J2 belonging to H. avenae. The PCR was able to detect up to 1:10 dilution of the DNA obtained from five J2. The results showed that a specific and sensitive H. latipons species-specific PCR was constructed.
536			_aGlobal Wheat Program
546			_aText in English
591			_aCIMMYT Informa No. 1860
594			_aINT2918|INT2410
595			_aCSC
650		7	_aActin _2AGROVOC _930707
650		7	_aGenes _2AGROVOC _93563
650		7	_aDiagnosis _2AGROVOC _95807
650		7	_aNucleotide sequence _2AGROVOC _91937
650		7	_aGenetic markers _2AGROVOC _91848
700	1		_9363 _aWaeyenberge, L.
700	1		_9358 _aViaene, N.
700	1		_aDababat, A.A. _9874 _8INT2918 _gGlobal Wheat Program
700	1		_aNicol, J.M. _9228
700	1		_aOgbonnaya, F.C. _9237
700	1		_aMoens, M. _9212
773	0		_tNematology _gv. 15, no. 6, p. 709-717 _dLeiden (Netherlands) : Brill, 2013. _w57516 _x1568-5411
856	4		_uhttps://hdl.handle.net/20.500.12665/596 _yAccess only for CIMMYT Staff
942			_cJA _2ddc _n0
999			_c30108 _d30108