000			03317nab a22003977a 4500
001			G97966
003			MX-TxCIM
005			20211123225507.0
008			211123s2013 gw |||p|op||| 00| 0 eng d
022			_a1432-2242 (Online)
022			_a0040-5752
024	8		_ahttps://doi.org/10.1007/s00122-013-2124-7
040			_aMX-TxCIM
041			_aeng
090			_aCIS-7171
100	0		_aHuixin Zhou _917335
245	1	0	_aMolecular mapping of leaf rust resistance gene LrNJ97 in Chinese wheat line Neijiang 977671
260			_aBerlin (Germany) : _bSpringer, _c2013.
500			_aPeer review
500			_aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0040-5752
520			_aNeijiang 977671 and 19 near-isogenic lines with known leaf rust resistance genes were inoculated with 12 pathotypes of Puccinia triticina for postulation of leaf rust resistance genes effective at the seedling stage. The reaction pattern of Neijiang 977671 differed from those of the lines with known leaf rust resistance genes used in the test, indicating that Neijiang 977671 may carry a new leaf rust resistance gene(s). With the objective of identifying and mapping the new gene for resistance to leaf rust, F1 and F2 plants, and F2:3 families, from Neijiang 977671 × Zhengzhou 5389 (susceptible) were inoculated with Chinese P. triticina pathotype FHNQ in the greenhouse. Results from the F2 and F2:3 populations indicated that a single dominant gene, temporarily designated LrNJ97, conferred resistance. In order to identify other possible genes in Neijiang 977671 other eight P. triticina pathotypes avirulent on Neijiang 977671 were used to inoculate 25 F2:3 families. The results showed that at least three leaf rust resistance genes were deduced in Neijiang 977671. Bulked segregant analysis was performed on equal amounts of genomic DNA from 20 resistant and 20 susceptible F2 plants. SSR markers polymorphic between the resistant and susceptible bulks were used to analyze the F2:3 families. LrNJ97 was linked to five SSR loci on chromosome 2BL. The two closest flanking SSR loci were Xwmc317 and Xbarc159 at genetic distances of 4.2 and 2.2 cM, respectively. At present two designated genes (Lr50 and Lr58) are located on chromosome 2BL. In the seedling tests, the reaction pattern of LrNJ97 was different from that of Lr50. Lr50 and Lr58 were derived from T. armeniacum and Ae. triuncialis, respectively, whereas according to the pedigree of Neijiang 977671 LrNJ97 is from common wheat. Although seeds of lines with Lr58 were not available, it was concluded that LrNJ97 is likely to be a new leaf rust resistance gene.
536			_aGlobal Wheat Program
546			_aText in English
591			_aCIMMYT Informa No. 1844|Springer
594			_aINT2411
595			_aCSC
700	0		_aXianchun Xia _9377
700	1		_aHe Zhonghu _gGlobal Wheat Program _8INT2411 _9838
700	0		_aXing Li _911009
700	0		_aCuifen Wang _93340
700	0		_aZaifeng Li _93343
700	0		_aDaqun Liu _93344
773	0		_tTheoretical and Applied Genetics _gv. 126, no. 8, p. 2141-2147 _wG444762 _dBerlin (Germany) : Springer, 2013. _x0040-5752
856	4		_uhttps://hdl.handle.net/20.500.12665/1664 _yAccess only for CIMMYT Staff
942			_cJA _2ddc _n0
999			_c30026 _d30026