000			03135nab a22004457a 4500
001			G97675
003			MX-TxCIM
005			20230512173656.0
008			210809s2013 ne |||p|op||| 00| 0 eng d
022			_a1448-6032 (Online)
022	0		_a0815-3191
024	8		_ahttps://doi.org/10.1007/s13313-012-0177-8
040			_aMX-TxCIM
041			_aeng
090			_aCIS-7031
100	1		_aEllis, M.H. _920500
245	1	0	_aIdentification of a new Polerovirus (family Luteoviridae) associated with cotton bunchy top disease in Australia
260			_aNetherlands : _bSpringer, _c2013.
500			_aPeer review
500			_aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0815-3191
520			_aCotton bunchy top (CBT) disease has caused significant yield losses in Australia and is now managed by control of its vector, the cotton aphid (Aphis gossypii). Its mode of transmission and similarities in symptoms to cotton Blue Disease suggested it may also be caused by a luteovirus or related virus. Degenerate primers to conserved regions of the genomes of the family Luteoviridae were used to amplify viral cDNAs from CBT-affected cotton leaf tissue that were not present in healthy plants. Partial genome sequence of a new virus (Cotton bunchy top virus, CBTV) was obtained spanning part of the RNA-dependent-RNA-polymerase (RdRP), all of the coat protein and part of the aphid-transmission protein. CBTV sequences could be detected in viruliferous aphids able to transmit CBT, but not aphids from non-symptomatic plants, indicating that it is associated with the disease and may be the causal agent. All CBTV open-reading frames had their closest similarity to viruses of the genus Polerovirus. The partial RdRP had 90 % amino acid identity to the RdRP of Cotton leafroll dwarf virus (CLRDV) that causes cotton blue disease, while other parts of the genome were more similar to other poleroviruses. The sequence similarity and genome organization of CBTV suggest that it should be considered a new member of the genus Polerovirus. This partial genome sequence of CBTV opens up the possibility for developing diagnostic tests for detection of the virus in cotton plants, aphids and weeds as well as alternative strategies for engineering CBT resistance in cotton plants through biotechnology.
536			_aGlobal Wheat Program
546			_aText in English
594			_aINT3233
595			_aCSC
650		7	_aCotton _2AGROVOC _94017
650		7	_aAphidoidea _2AGROVOC _910531
650		7	_aViruses _2AGROVOC _91306
650		7	_aPolerovirus _2AGROVOC _930829
651		7	_2AGROVOC _98101 _aAustralia
700	1		_aSilva, T.F. _921981
700	1		_aStiller, W.N. _921970
700	1		_aWilson, L.J. _921982
700	1		_aVaslin, M.F.S. _921983
700	1		_aSharman, M. _921984
700	1		_aLlewellyn, D.J. _921985
773	0		_tAustralasian Plant Pathology _gv. 42, no. 3, p. 261-269 _dNetherlands : Springer, 2013. _wG445304 _x0815-3191
856	4		_uhttps://hdl.handle.net/20.500.12665/351 _yAccess only for CIMMYT Staff
942			_cJA _2ddc _n0
999			_c29850 _d29850