000 03132nab a22003977a 4500
001 G97597
003 MX-TxCIM
005 20231005213436.0
008 211111s2013 ii |||p|op||| 00| 0 eng d
022 _a0974-1275 (Online)
022 0 _a0971-7811
024 8 _ahttps://doi.org/10.1007/s13562-012-0167-5
040 _aMX-TxCIM
041 _aeng
090 _aCIS-6992
100 1 _aWasala, S.K.
_924893
245 1 0 _aMicrosatellite marker-based diversity and population genetic analysis of selected lowland and mid-altitude maize landrace accessions of India
260 _aIndia :
_bSpringer :
_bSociety for Plant Biochemistry and biotechnology,
_c2013.
500 _aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0971-7811
500 _aPeer review
520 _aMaize (Zea mays L.) harbours significant genetic diversity not only in its centre of origin (Mexico) but also in several countries worldwide, including India, in the form of landraces. In this study, DNA fingerprinting of 48 landrace accessions from diverse regions of India was undertaken using 42 fluorescent dye-labeled Simple Sequence Repeat (SSR) markers, followed by allele resolution using DNA sequencer and analysis of molecular diversity within and among these landraces. The study revealed a large number of alleles (550), with high mean number of alleles per locus (13.1), and Polymorphism Information Content (PIC) of 0.60, reflecting the level of diversity in the landrace accessions. Besides identification of 174 unique alleles in 44 accessions, six highly frequent SSR alleles were detected at six loci (phi014, phi090, phi112, umc1367, phi062 and umc1266) with individual frequencies greater than 0.75, indicating that chromosomal regions harboring these SSR alleles are not selectively neutral. F statistics revealed very high genetic differentiation, population subdivision and varying levels of inbreeding in the landraces. Analysis of Molecular Variance showed that 63 % of the total variation in the accessions could be attributed to within-population diversity, and 37 % represented between population diversity. Cluster analysis of SSR data using Nei?s genetic distance and UPGMA revealed considerable genetic diversity in these populations, although no clear separation of accessions was observed based on their geographic origin.
536 _aGlobal Maize Program
546 _aText in English
591 _aCIMMYT Informa No. 1825|Springer
594 _aINT3057
595 _aCSC
650 7 _aGenetic variation
_2AGROVOC
_91129
650 7 _aLandraces
_2AGROVOC
_96305
650 7 _aPopulation Structure
_2AGROVOC
_98720
650 7 _aZea mays
_2AGROVOC
_91314
651 7 _2AGROVOC
_93726
_aIndia
700 1 _aPrasanna, B.M.
_gGlobal Maize Program
_8INT3057
_9887
773 0 _tJournal of Plant Biochemistry and Biotechnology
_gv. 22, no. 4, p. 392-400
_x0971-7811
_dIndia : Springer : Society for Plant Biochemistry and biotechnology, 2013.
856 4 _uhttps://hdl.handle.net/20.500.12665/832
_yAccess only for CIMMYT Staff
942 _cJA
_2ddc
_n0
999 _c29788
_d29788