000 02669nab a22003857a 4500
001 G97443
003 MX-TxCIM
005 20171220113603.0
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024 8 _ahttps://doi.org/10.1111/j.1439-0434.2012.01956.x
040 _aMX-TxCIM
041 0 _aEn
100 1 _aGyawali, S.
245 0 0 _aGenetic structure of Cochliobolus sativus populations sampled from roots and leaves of barley and wheat in North Dakota
260 _c2012
500 _aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0931-1785
520 _aCommon root rot (CRR) and spot blotch, caused by Cochliobolus sativus (Ito and Kurib.) Drechsl. ex Dast., are important diseases of barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.) worldwide. However, the population biology of C. sativus is still poorly understood. In this study, the genetic structure of three C. sativus populations, consisting of isolates sampled respectively from barley leaves (BL), barley roots (BR) and wheat roots (WR) in North Dakota, was analysed with amplified fragment length polymorphism (AFLP) markers. A total of 127 AFLP loci were generated among 208 C. sativus isolates analysed with three primer combinations. Gene diversity (H = 0.277?0.335) were high in all three populations. Genetic variation among C. sativus individuals within population accounted for 74%, whereas 26% of the genetic variation was explained among populations. Genetic differentiation was high (ØPT = 0.261, corrected = 0.39), whereas gene flow (Nm) ranged from 1.27 to 1.56 among the three populations analysed. The multilocus linkage disequilibrium (LD) (= 0.076?0.117) was moderate in C sativus populations. Cluster analyses indicate that C. sativus populations differentiated according to the hosts (barley and wheat) and tissues (root and leaf) although generalists also exist in North Dakota. Crop breeding may benefit from combining genes for resistance against both specialists and generalists of C. sativus.
546 _aEnglish
593 _aCarelia Juarez
595 _aRPC
650 1 0 _aAmplified fragment length polymorphism
650 1 0 _aBarley
_91018
650 1 0 _aCochliobolus sativus
_92193
650 1 0 _aCommon root rot
650 1 0 _aGenetic differentiation
650 1 0 _aSpot blotch
650 1 0 _aWheat
700 1 _aAdhikari, T.B.,
_ecoaut.
700 1 _aBurlakoti, R.R.,
_ecoaut.
700 1 _aNeate, S.M.,
_ecoaut.
700 1 _aPuri, K.D.,
_ecoaut.
700 1 _aShaobin Zhong,
_ecoaut.
773 0 _tJournal of Phytopathology
_gv. 160, no. 11-12, p. 637-646
942 _cJA
999 _c29708
_d29708