| 000 | 02405nab a22003977a 4500 | ||
|---|---|---|---|
| 001 | G96930 | ||
| 003 | MX-TxCIM | ||
| 005 | 20190717174555.0 | ||
| 008 | 121211b |||p||p||||||| |z||| | | ||
| 022 | _a1432-2048 (Revista en electrónico) | ||
| 022 | 0 | _a0032-0935 | |
| 024 | 8 | _ahttps://doi.org/10.1007/s00425-011-1577-7 | |
| 040 | _aMX-TxCIM | ||
| 041 | 0 | _aEn | |
| 090 | _aREP-13294 | ||
| 100 | 1 |
_9957 _aGuzman, C. _gGlobal Wheat Program _8INT3466 |
|
| 245 | 0 | 0 | _aMolecular characterization of a new waxy allele with partial expression in spelt wheat |
| 260 | _c2012 | ||
| 500 | _aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0032-0935 | ||
| 520 | _aStarch composition which is dependent on the waxy protein, the enzyme responsible for amylose synthesis in the grain, is an important aspect of the wheat quality. In this report, we describe the characterization of a novel Wx-A1 allele (Wx-A1g formerly known as -Wx-A1a) in Spanish spelt wheat lines which is responsible for a remarkable decline in the concentration of Wx-A1 protein found in the endosperm. Comparison of the DNA sequences in the Wx-A1a and Wx-A1g alleles showed the presence of a 160-bp insertion within the fourth intron in the latter. This insertion had some characteristics of a transposable-like element. RT-PCR analysis showed the presence of normal and aberrant mRNA transcripts in the Wx-A1g lines, indicating that the aberrant transcripts are un-spliced and contained the longer fourth intron. This may be related to the low level of Wx-A1 protein in these lines. In addition, a simple and fast PCR assay was designed for differentiating among different Wx-A1 alleles (a, b, f and g). The mutation described here is not related to either of the Wx-A1 mutations identified previously in common and durum wheats and could help to extend the range of amylose content of wheats. | ||
| 536 | _aGlobal Wheat Program | ||
| 546 | _aEnglish | ||
| 591 | _aNo CIMMYT affiliation | ||
| 593 | _aCarelia Juarez | ||
| 594 | _aINT3466 | ||
| 595 | _aRPC | ||
| 650 | 1 | 0 | _aNull mutations |
| 650 | 1 | 0 | _aSpelt wheat |
| 650 | 1 | 0 | _aStarch |
| 650 | 1 | 0 | _aWaxy proteins |
| 700 | 1 |
_aAlvarez, J.B., _ecoaut. _92175 |
|
| 700 | 1 |
_aCaballero, L., _ecoaut. |
|
| 700 | 1 |
_aYamamori, M., _ecoaut. _92176 |
|
| 773 | 0 |
_tPlanta _gv. 235, no. 6, p. 1331-1339 |
|
| 942 | _cJA | ||
| 999 |
_c29354 _d29354 |
||