000 01932nab a22002897a 4500
001 G96747
003 MX-TxCIM
005 20171220113549.0
008 121211b |||p||p||||||| |z||| |
024 8 _ahttps://doi.org/10.1111/j.1439-037X.1992.tb01029.x
040 _aMX-TxCIM
041 0 _aEn
100 1 _aZhang, Q.Z.
245 0 0 _aA practical application of starch determination in vegetative plant material
260 _c1992
500 _aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0931-2250
520 _aStarch determination in plant matter is necessary because of the importance of the carbohydrate pool in combination of the nitrogen pool in crop growth and development. This analysis is complicated in vegetative materials by contamination with non-starch containing material. Starch is not a homogenous material, so the content of amylose and amylopectin varies from the different growth stages and from crop to crop. Therefore standard methods of starch estimation give no exact result. The method described here based on a pretreatment of the plant material with dilute alkali before hydrolysis with amyloglucosidase and the estimation of the glucose content. Therefore this procedure was first tested on amylose, amylopectin, soluble starch, three commercially available starches, and was then applied to vegetative rape material. Recoveries of amylose and three commercial starches were between 98 and 100 % after a additional ultrasonic treatment. The method is advantageous because it is simple, quick and reproducible.
546 _aEnglish
593 _aCarelia Juarez
595 _aRPC
650 1 0 _aStarch determination
650 1 0 _aVegetative plant material
700 1 _aGreef, J.M.,
_ecoaut.
700 1 _aKullmann, A.,
_ecoaut.
773 0 _tJournal of Agronomy and Crop Science
_gv. 169, no. 4, p. 217-222
942 _cJA
999 _c29222
_d29222