| 000 | 02213nab a22003017a 4500 | ||
|---|---|---|---|
| 001 | G96416 | ||
| 003 | MX-TxCIM | ||
| 008 | 121211b |||p||p||||||| |z||| | | ||
| 040 | _aMX-TxCIM | ||
| 041 | 0 | _aEn | |
| 043 | _aUS | ||
| 100 | 1 | _aVersalovic, J. | |
| 245 | 0 | 0 | _aGenomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction |
| 260 | _c1994 | ||
| 520 | _aInterspersed repetitive sequences in prokaryotic genomes can be used as oligonucleotide primer binding sites for polymerase chain reaction (PCR)-mediated genomic fingerprinting (rep-PCR). rep-PCR is based on the observation that outwardly facing oligonucleotide primers, complementary to interspersed repeated sequences, enable the amplification of differently sized DNA fragments, consisting of sequences lying between these elements. Multiple amplicons of different sizes can be fractionated by electrophoresis and enable the establishment of DNA fingerprint patterns specific for individual bacterial strains. Several of these interspersed repetitive elements are conserved in diverse genera of bacteria and, therefore, enable single primer sets to be used for DNA fingerprinting many different microorganisms. Purified genomic DNA, crude bacterial cell lysates, or infected samples can be used directly in rep-PCR to generate DNA fingerprint profiles for comparative analyses. Detection and analysis of differently sized rep-PCR products include visual inspection of ethidium bromide-stained gels, computer-assisted detection and data storage methods, or laser scanning detection of fluorophore-labeled amplification products. DNA fingerprint patterns can be compared to estimate relative degrees of similarity between isolates and help determine whether isolates are clonally related. | ||
| 546 | _aEnglish | ||
| 593 | _aCarelia Juarez | ||
| 595 | _aRPC | ||
| 650 | 1 | 0 |
_aBacteria _91017 |
| 650 | 1 | 0 | _aDna fingerprinting |
| 650 | 1 | 7 |
_aGenomes _gAGROVOC _2 _91131 |
| 650 | 1 | 0 | _aPolymerase chain reaction |
| 700 | 1 |
_aBruijn, F.J. de, _ecoaut. |
|
| 700 | 1 |
_aLupski, J.R., _ecoaut. |
|
| 700 | 1 |
_aSchneider, M., _ecoaut. |
|
| 773 | 0 |
_tMethods in Molecular and Celluler Biology _gv. 5, no. 1, p. 25-40 |
|
| 942 | _cJA | ||
| 999 |
_c29063 _d29063 |
||