000			03455nab a22004577a 4500
001			G95937
003			MX-TxCIM
005			20230522235622.0
008			211110s2012 xxu|||p|op||| 00| 0 eng d
022			_a1943-2631 (Online)
022			_a0016-6731
024	8		_ahttps://doi.org/10.1534/genetics.111.133066
040			_aMX-TxCIM
041			_aeng
090			_aCIS-7136
100	1		_aPrigge, V. _924871
245	1	0	_aNew insights into the genetics of in vivo induction of maternal haploids, the backbone of doubled haploid technology in maize
260			_aUSA : _bGenetics Society of America, _c2012.
500			_aPeer review
500			_aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=1943-2631
520			_aHaploids and doubled haploid (DH) inbred lines have become an invaluable tool for maize genetic research and hybrid breeding, but the genetic basis of in vivo induction of maternal haploids is still unknown. This is the first study reporting comparative quantitative trait locus (QTL) analyses of this trait in maize. We determined haploid induction rates (HIR) in testcrosses of a total of 1061 progenies of four segregating populations involving two temperate haploid inducers, UH400 (HIR=8%) and CAUHOI (HIR=2%), one temperate and two tropical inbreds with HIR=0%, and up to three generations per population. Mean HIR of the populations ranged from 0.6 to 5.2% and strongly deviated from the mid-parent values. One QTL (qhir1) explaining up to p^ = 66% of the genetic variance was detected in bin 1.04 in the three populations involving a non-inducer parent and the HIR-enhancing allele was contributed by UH400. Segregation ratios of loci in bin 1.04 were highly distorted against the UH400 allele in these three populations, suggesting that transmission failure of the inducer gamete and haploid induction ability are related phenomena. In the CAUHOIxUH400 population, seven QTL were identified on five chromosomes, with qhir8 on chromosome having p^ > 20% in three generations of this cross. The large-effect QTL qhir1 and qhir8 will likely become fixed quickly during inducer development due to strong selection pressure applied for high HIR. Hence, marker-based pyramiding of small-effect and/or modifier QTL influencing qhir1 and qhir8 may help to further increase HIR in maize. We propose a conceptual genetic framework for inheritance of haploid induction ability, which is also applicable to other dichotomous traits requiring progeny testing, and discuss the implications of our results for haploid inducer development.
536			_aGlobal Maize Program
546			_aText in English
591			_aCIMMYT Informa No. 1779
594			_aINT2925
595			_aCSC
650		7	_aGenetics _91130 _2AGROVOC
650		7	_aIn vivo experimentation _930881 _2AGROVOC
650		7	_aHaploidy _91925 _2AGROVOC
650		7	_aMaize _91173 _2AGROVOC
650		7	_aBiotechnology _95143 _2AGROVOC
700	0		_aXiaowei Xu _916844
700	0		_aLiang Li _916845
700	1		_9875 _aBABU, R. _gGlobal Maize Program _8INT2925
700	0		_aShaojiang Chen _93484
700	1		_92252 _aAtlin, G.N.
700	1		_aMelchinger, A.E. _93373
773	0		_tGenetics _gv. 190, no. 2, p. 781-793 _x0016-6731 _wG444332 _dUSA : Genetics Society of America, 2012.
856	4		_uhttps://hdl.handle.net/20.500.12665/931 _yAccess only for CIMMYT Staff
942			_cJA _2ddc _n0
999			_c28828 _d28828