| 000 | 03442nab a22004817a 4500 | ||
|---|---|---|---|
| 001 | G95715 | ||
| 003 | MX-TxCIM | ||
| 005 | 20230105211133.0 | ||
| 008 | 220505s2012 gw |||p|op||| 00| 0 eng d | ||
| 022 | _a1432-2242 (Online) | ||
| 022 | 0 | _a0040-5752 | |
| 024 | 8 | _ahttps://doi.org/10.1007/s00122-011-1759-5 | |
| 040 | _aMX-TxCIM | ||
| 041 | 0 | _aeng | |
| 090 | _aCIS-7080 | ||
| 100 | 1 |
_aVan Schalkwyk, A. _927350 |
|
| 245 | 1 | 0 | _aBin mapping of tomato diversity array (DArT) markers to genomic regions of Solanum lycopersicum x Solanum pennellii introgression lines |
| 260 |
_aBerlin (Germany) : _bSpringer, _c2012. |
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| 500 | _aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0040-5752 | ||
| 500 | _aPeer review | ||
| 500 | _aOpen Access | ||
| 520 | _aMarker-trait association studies in tomato have progressed rapidly due to the availability of several populations developed between wild species and domesticated tomato. However, in the absence of whole genome sequences for each wild species, molecular marker methods for whole genome comparisons and fine mapping are required. We describe the development and validation of a diversity arrays technology (DArT) platform for tomato using an introgression line (IL) population consisting of wild Solanum pennellii introgressed into Solanum lycopersicum (cv. M82). A tomato diversity array consisting of 6,912 clones from domesticated tomato and twelve wild tomato/Solanaceous species was constructed. We successfully bin-mapped 990 polymorphic DArT markers together with 108 RFLP markers across the IL population, increasing the number of markers available for each S. pennellii introgression by tenfold on average. A subset of DArT markers from ILs previously associated with increased levels of lycopene and carotene were sequenced, and 44% matched protein coding genes. The bin-map position and order of sequenced DArT markers correlated well with their physical position on scaffolds of the draft tomato genome sequence (SL2.40). The utility of sequenced DArT markers was illustrated by converting several markers in both the S. pennellii and S. lycopersicum phases to cleaved amplified polymorphic sequence (CAPS) markers. Genotype scores from the CAPS markers confirmed the genotype scores from the DArT hybridizations used to construct the bin map. The tomato diversity array provides additional "sequence-characterized" markers for fine mapping of QTLs in S. pennellii ILs and wild tomato species. | ||
| 536 | _aGenetic Resources Program | ||
| 546 | _aText in English | ||
| 591 | _aSpringer | ||
| 594 | _aINT3049 | ||
| 650 | 7 |
_97173 _aSolanum lycopersicum _2AGROVOC |
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| 650 | 7 |
_99140 _aSolanum pennellii _2AGROVOC |
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| 650 | 7 |
_91848 _aGenetic markers _2AGROVOC |
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| 650 | 7 |
_98730 _aIntrogression _2AGROVOC |
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| 650 | 7 |
_91134 _aGenotypes _2AGROVOC |
|
| 650 | 0 |
_aDNA Sequences _gAGROVOC _98666 |
|
| 700 | 1 |
_9885 _aWenzl, P. _gGenetic Resources Program _8INT3049 |
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| 700 | 1 |
_aSmit, S. _927351 |
|
| 700 | 1 |
_aLopez-Cobollo, R. _927352 |
|
| 700 | 1 |
_aKilian, A. _91409 |
|
| 700 | 1 |
_aBishop, G. _927353 |
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| 700 | 1 |
_aHefer, C. _927354 |
|
| 700 | 1 |
_aBerger, D.K. _918997 |
|
| 773 | 0 |
_tTheoretical and Applied Genetics _gv. 124, no. 5, p. 947-956 _dBerlin (Germany) : Springer, 2012. _wG444762 _x0040-5752 |
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| 856 | 4 |
_yOpen Access through DSpace _uhttp://hdl.handle.net/10883/1866 |
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| 942 |
_cJA _2ddc _n0 |
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| 999 |
_c28726 _d28726 |
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