000			02735nab a22003977a 4500
001			G95584
003			MX-TxCIM
005			20230331215652.0
008			211019s2011 xxu|||p|op||| 00| 0 eng d
022	0		_a0733-5210
024	8		_ahttps://doi.org/10.1016/j.jcs.2011.09.004
040			_aMX-TxCIM
041			_aeng
090			_aCIS-6395
100	1		_aRam, S. _917641
245	1	0	_aComparative analyses of LMW glutenin alleles in bread wheat using allele-specific PCR and SDS-PAGE
260			_aUSA : _bElsevier, _c2011.
500			_aPeer review
500			_aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0733-5210
520			_aOne hundred and eighty-two bread wheat cultivars developed in India were characterized for low molecular weight (LMW) glutenins using SDS-PAGE and allele-specific polymerase chain reaction (PCR) to assess allelic diversity encoded by Glu-3 loci, as well as their utility for correctly identifying different alleles. SDS-PAGE indicated Glu-A3c is present in 64.6% of the cultivars, Glu-A3b in 13.8%, Glu-A3d in 12.7% and Glu-A3e/f in 8.8%. Seven types of alleles were present at the Glu-B3 locus: Glu-B3b (29.3%), Glu-B3g (27.0%), Glu-B3h (13.8%), Glu-B3i (16.1%), Glu-B3j (12.1%), Glu-B3c (0.6%) and Glu-B3d (1.1%). SDS-PAGE found three types of Glu-D3 alleles: Glu-D3a (30.2%), Glu-D3b (67.1%) and Glu-D3c (2.7%). However, PCR found two different alleles in cultivars classified as carrying Glu-D3a and three alleles in those identified as carrying Glu-D3b cultivars, indicating a more complex nature of the Glu-D3 locus. In conclusion, the data found greater consistency between the SDS-PAGE and PCR amplification patterns of alleles such as Glu-A3c, Glu-A3d, Glu-B3g, Glu-B3h and Glu-B3i, and less consistency between those same patterns in the Glu-A3b, Glu-A3e/f and Glu-B3b alleles. More studies are needed in order to achieve unambiguous identification of the Glu-3 alleles and thereby allow their greater utility in germplasm evaluation and breeding.
536			_aGlobal Wheat Program
546			_aText in English
591			_aCIMMYT Informa No. 1775|Elsevier
594			_aINT0368
595			_aCSC
650		7	_aPCR _2AGROVOC _912563
650		7	_aWheat _2AGROVOC _91310
650		7	_91137 _aGlutenins _2AGROVOC
700	1		_aSharma, S. _924122
700	1		_aVerma, A. _924123
700			_aTyagi, B.S. _910074
700	1		_aPeña-Bautista, R.J. _8INT0368 _gGlobal Wheat Program _9645
773	0		_tJournal of Cereal Science _gv. 54, no. 3, p. 488-493 _dUSA : Elsevier, 2011. _x0733-5210 _wG444514
856	4		_uhttps://hdl.handle.net/20.500.12665/1435 _yAccess only for CIMMYT Staff
942			_cJA _2ddc _n0
999			_c28698 _d28698