000			03127nab a22004457a 4500
001			G95372
003			MX-TxCIM
005			20221118224145.0
008			221118s2011 xxu|||p|op||| 00| 0 eng d
022			_a1932-6203 (Online)
024	8		_ahttps://doi.org/10.1371/journal.pone.0024699
040			_aMX-TxCIM
041			_aeng
090			_aCIS-6360
100	0		_aLin Li _922830
245	1	0	_aAn 11-bp insertion in Zea mays fatb reduces the palmitic acid content of fatty acids in maize grain
260			_aSan Francisco, CA (USA) : _bPublic Library of Science, _c2011.
500			_aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=1932-6203
500			_aPeer review
500			_aOpen Access
520			_aThe ratio of saturated to unsaturated fatty acids in maize kernels strongly impacts human and livestock health, but is a complex trait that is difficult to select based on phenotype. Map-based cloning of quantitative trait loci (QTL) is a powerful but time-consuming method for the dissection of complex traits. Here, we combine linkage and association analyses to fine map QTL-Pal9, a QTL influencing levels of palmitic acid, an important class of saturated fatty acid. QTL-Pal9 was mapped to a 90-kb region, in which we identified a candidate gene, Zea mays fatb (Zmfatb), which encodes acyl-ACP thioesterase. An 11-bp insertion in the last exon of Zmfatb decreases palmitic acid content and concentration, leading to an optimization of the ratio of saturated to unsaturated fatty acids while having no effect on total oil content. We used three-dimensional structure analysis to explain the functional mechanism of the ZmFATB protein and confirmed the proposed model in vitro and in vivo. We measured the genetic effect of the functional site in 15 different genetic backgrounds and found a maximum change of 4.57 mg/g palmitic acid content, which accounts for ~20?60% of the variation in the ratio of saturated to unsaturated fatty acids. A PCR-based marker for QTL-Pal9 was developed for marker-assisted selection of nutritionally healthier maize lines. The method presented here provides a new, efficient way to clone QTL, and the cloned palmitic acid QTL sheds lights on the genetic mechanism of oil biosynthesis and targeted maize molecular breeding.
546			_aText in English
650		7	_aMaize _2AGROVOC _91173
650		7	_99144 _aPalmitic Acid _2AGROVOC
650		7	_92084 _aChromosome mapping _2AGROVOC
650		7	_98903 _aChromosome manipulation _2AGROVOC
700	0		_aHui Li _918792
700	0		_aQing Li _917870
700	0		_aXiaoHong Yang _919531
700	0		_aDebo Zheng _929119
700	1		_aWarburton, M.L. _94138
700	0		_aYuchao Chai _921871
700	0		_aPan Zhang _929120
700	0		_aYuqiu Guo _924858
700	1		_9398 _aJianbing Yan
700	0		_aJianSheng Li _919532
773	0		_tPLoS ONE _gv. 6, no. 9, e24699 _dSan Francisco, CA (USA) : Public Library of Science, 2011. _wG94957 _x1932-6203
856	4		_yOpen Access through DSpace _uhttp://hdl.handle.net/10883/1853
942			_cJA _2ddc _n0
999			_c28595 _d28595