000			02955nab a22003857a 4500
001			G94896
003			MX-TxCIM
005			20220704150302.0
008			220704s2010 xxu|||p|op||| 00| 0 eng d
022			_a1940-3372 (Online)
024	8		_ahttps://doi.org/10.3835/plantgenome2010.06.0013
040			_aMX-TxCIM
041			_aeng
090			_aCIS-6280
100	1		_aCórdoba, J.M. _927966
245	1	0	_aIdentification and mapping of simple sequence repeat markers from common bean (phaseolus vulgaris l.) bacterial artificial chromosome end sequences for genome characterization and genetic-physical map integration
260			_aMadison, WI (USA) : _bCSSA : _bWiley, _c2010.
500			_aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=1940-3372
500			_aPeer review
500			_aOpen Access
520			_aMicrosatellite markers or simple sequence repeat (SSR) loci are useful for diversity characterization and genetic?physical mapping. Different in silico microsatellite search methods have been developed for mining bacterial artificial chromosome (BAC) end sequences for SSRs. The overall goal of this study was genome characterization based on SSRs in 89,017 BAC end sequences (BESs) from the G19833 common bean (Phaseolus vulgaris L.) library. Another objective was to identify new SSR taking into account three tandem motif identification programs (Automated Microsatellite Marker Development [AMMD], Tandem Repeats Finder [TRF], and SSRLocator [SSRL]). Among the microsatellite search engines, SSRL identified the highest number of SSRs; however, when primer design was attempted, the number dropped due to poor primer design regions. Automated Microsatellite Marker Development software identified many SSRs with valuable AT/TA or AG/TC motifs, while TRF found fewer SSRs and produced no primers. A subgroup of 323 AT-rich, di-, and trinucleotide SSRs were selected from the AMMD results and used in a parental survey with DOR364 and G19833, of which 75 could be mapped in the corresponding population; these represented 4052 BAC clones. Together with 92 previously mapped BES- and 114 non-BES-derived markers, a total of 280 SSRs were included in the polymerase chain reaction (PCR)-based map, integrating a total of 8232 BAC clones in 162 contigs from the physical map.
546			_aText in English
595			_aCSC
650		7	_99039 _aPhaseolus vulgaris _2AGROVOC
650		7	_91848 _aGenetic markers _2AGROVOC
650		7	_92261 _aMicrosatellites _2AGROVOC
650		7	_94190 _aGenetic maps _2AGROVOC
700	1		_aChavarro, C. _927967
700	1		_aRojas, F. _927968
700	1		_aMuñoz, C. _927969
700	1		_aBlair, M.W. _921809
773	0		_tPlant Genome _gv. 3, no. 3, p. 154-165 _dMadison, WI (USA) : CSSA : Wiley, 2010. _wG94757 _x1940-3372
856	4		_uhttp://hdl.handle.net/10883/1845 _yOpen Access through DSpace
942			_cJA _2ddc _n0
999			_c28370 _d28370