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022 0 _a0022-0957
024 8 _ahttps://doi.org/10.1093/jxb/erq414
040 _aMX-TxCIM
100 1 _aCaldelas, C.
245 0 0 _aZinc isotopic fractionation in Phragmites australis in response to toxic levels of zinc
260 _c2010 ?
500 _aIn press
500 _aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0022-0957
520 _aStable isotope signatures of Zn have shown great promise in elucidating changes in uptake and translocation mechanisms of this metal in plants during environmental changes. Here this potential was tested by investigating the effect of high Zn concentrations on the isotopic fractionation patterns of Phragmites australis (Cav.) Trin. Ex Steud. Plants were grown for 40 d in a nutritive solution containing 3.2 mM (sufficient) or 2 mM (toxic) Zn. The Zn isotopic composition of roots, rhizomes, shoots, and leaves was analysed. Stems and leaves were sampled at different heights to evaluate the effect of long-distance transport on Zn fractionation. During Zn sufficiency, roots, rhizomes, and shoots were isotopically heavy (d66ZnJMC Lyon¼0.2&) while the youngest leaves were isotopically light (?0.5&). During Zn excess, roots were still isotopically heavier (d66Zn¼0.5&) and the rest of the plant was isotopically light (up to ?0.5&). The enrichment of heavy isotopes at the roots was attributed to Zn uptake mediated by transporter proteins under Zn-sufficient conditions and to chelation and compartmentation in Zn excess. The isotopically lighter Zn in shoots and leaves is consistent with long-distance root to shoot transport. The tolerance response of P. australis increased the range of Zn fractionation within the plant and with respect to the environment.
546 _aEnglish
591 _aOxford
593 _aLucia Segura
595 _aRPC
650 1 0 _aIsotope fractionation
650 1 0 _aMC-ICP-MS
650 1 0 _ametallomics
650 1 0 _aMetals
650 1 0 _aNutrition
650 1 0 _aPhragmites australis
650 1 0 _areed
700 1 _91436
_aAraus, J.L.
700 1 _aDong, S.,
_ecoaut.
700 1 _aWeiss, D.J.,
_ecoaut.
773 0 _tJournal of Experimental Botany
942 _cJA
999 _c28335
_d28335