000			03206nab a22004697a 4500
001			G94554
003			MX-TxCIM
005			20230728224238.0
008			210805t2010 gw |||p|op||| 00| 0 eng d
022			_a1432-2242 (Online)
022			_a0040-5752
024	8		_ahttps://doi.org/10.1007/s00122-010-1323-8
040			_aMX-TxCIM
041			_aeng
090			_aCIS-6125
100	1		_9885 _aWenzl, P. _gGenetic Resources Program _8INT3049
245	1	0	_aIsolated chromosomes as a new and efficient source of DArT markers for the saturation of genetic maps
260			_aBerlin (Germany) : _bSpringer, _c2010.
500			_aPeer review
500			_aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0040-5752
520			_aWe describe how the diversity arrays technology (DArT) can be coupled with chromosome sorting to increase the density of genetic maps in specific genome regions. Chromosome 3B and the short arm of chromosome 1B (1BS) of wheat were isolated by flow cytometric sorting and used to develop chromosome- and chromosome arm-enriched genotyping arrays containing 2,688 3B clones and 384 1BS clones. Linkage analysis showed that 553 of the 711 polymorphic 3B-derived markers (78%) mapped to chromosome 3B, and 59 of the 68 polymorphic 1BS-derived markers (87%) mapped to chromosome 1BS, confirming the efficiency of the chromosome-sorting approach. To demonstrate the potential for saturation of genetic maps, we constructed a consensus map of chromosome 3B using 19 mapping populations, including some that were genotyped with the 3B-enriched array. The 3B-derived DArT markers doubled the number of genetic loci covered. The resulting consensus map, probably the densest genetic map of 3B available to this date, contains 939 markers (779 DArTs and 160 other markers) that segregate on 304 genetically distinct loci. Importantly, only 2,688 3B-derived clones (probes) had to be screened to obtain almost twice as many polymorphic 3B markers (510) as identified by screening approximately 70,000 whole genome-derived clones (269). Since an enriched DArT array can be developed from less than 5 ng of chromosomal DNA, a quantity which can be obtained within 1 h of sorting, this approach can be readily applied to any crop for which chromosome sorting is available.
536			_aGenetic Resources Program
546			_aText in English
591			_aSpringer
594			_aINT3049
650		7	_2AGROVOC _91130 _aGenetics
650		7	_2AGROVOC _92084 _aChromosome mapping
650		7	_2AGROVOC _94190 _aGenetic maps
700	1		_921890 _aSuchánková, P.
700	1		_9456 _aCarling, J.
700	1		_921891 _aŠimková, H.
700	1		_910451 _aHuttner, E.
700	1		_921892 _aKubaláková, M.
700	1		_921893 _aSourdille, P.
700	1		_921894 _aPaul, E.
700	1		_95160 _aFeuillet, C.
700	1		_91409 _aKilian, A.
700	1		_921895 _aDoležel, J.
773	0		_tTheoretical and Applied Genetics _gv. 121, no. 3, p. 465-474 _dBerlin (Germany) : Springer, 2010. _wG444762 _x0040-5752
856	4		_yAccess only for CIMMYT Staff _uhttps://hdl.handle.net/20.500.12665/287
942			_cJA _2ddc _n0
999			_c28293 _d28293