000			03194nab a22003737a 4500
001			G94538
003			MX-TxCIM
005			20230731154935.0
008			210706s2010 cc |||p|op||| 00| 0 chi d
022	0		_a0253-9772
024	8		_ahttps://doi.org/10.3724/SP.J.1005.2010.00613
040			_aMX-TxCIM
041			_achi
090			_aCIS-6113
100	0		_aLin-hai Wang _920836
245	1	0	_aCloning and phylogenetic analysis of low-molecular-weight glutenin subunit genes at Glu-B3 locus in common wheat relative species
260			_aBeijing (China) : _bScience Press, _c2010.
500			_aPeer review
500			_aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0253-9772
520			_aThe common wheat relative species are important germplasm for wheat breeding. In the present study, novel allelic variants at Glu-B3 locus were cloned to provide gene resources for wheat quality improvement. Four Glu-B3-locus specific primer sets LB1F/LB1R, LB2F/LB2R, LB3F/LB3R, and LB4F/LB4R were employed to isolate novel allelic variants of GluB3-1, GluB3-2, GluB3-3, and GluB3-4 from seven common wheat relative species, i.e., T. durum, T. dicoccum, T. dicoccoides, Aegilops longissima, Ae. searsii, Ae. Bicornis, and Ae. speltoides, and the software MEGA 4 was used to construct a phylogenetic tree. In total, 16 novel allelic variants of GluB3-1, GluB3-3, and GluB3-4 genes were isolated from the seven common wheat relative species, designated GluB3-16, GluB3-35, GluB3-36, GluB3-37, GluB3-46, GluB3-47, GluB3-48, GluB3-49, GluB3-410, GluB3-411, GluB3-412, GluB3-413, GluB3-414, GluB3-415, GluB3-416 and GluB3-417, respectively. In detail, GluB3-16 was cloned from T. dicoccoides with LB1F/LB1R, and the molecular weight of the deduced amino acid was 39.2 kDa. GluB3-35, GluB3-36, and GluB3-37 were isolated from T. durum and T. dicoccum with the primer set LB3F/LB3R, and the molecular weights of their deduced peptides were 44.5 kDa (GluB3-36) and 44.6 kDa (GluB3-35 and GluB3-37). The molecular weight of deduced peptides of GluB3-4 ranged from 38.6 kDa (GluB3-414) to 42.5 kDa (GluB3-413). All the 16 new allelic variants showed a single open reading frame (ORF), and their deduced amino-acid sequences had a typical sequence structure of LMW-GS. The allelic variants at Glu-B3 locus identified in common wheat relative species provide potential gene resources for wheat quality breeding and gene transformation. The results suggested that these Glu-B3 genes originated from different evolution processes.
536			_aGlobal Wheat Program
546			_aText in Chinese
594			_aINT2411
650		7	_aTriticum aestivum _2AGROVOC _91296
650		7	_aGlutenins _2AGROVOC _91137
650		7	_aPhylogenetics _2AGROVOC _930107
700	0		_aZhou Min _920928
700	0		_aLi Hui-Ling _920929
700	1		_aHe Zhonghu _gGlobal Wheat Program _8INT2411 _9838
700	0		_9377 _aXianchun Xia
773	0		_tHereditas (Beijing) _gv. 32, no. 6, p. 613-624 _dBeijing (China) : Science Press, 2010. _x0253-9772
856	4		_yAccess only for CIMMYT Staff _uhttps://hdl.handle.net/20.500.12665/704
942			_cJA _2ddc _n0
999			_c28280 _d28280