| 000 | 02474nab a22004097a 4500 | ||
|---|---|---|---|
| 001 | G94526 | ||
| 003 | MX-TxCIM | ||
| 005 | 20230731162002.0 | ||
| 008 | 210706s2010 at |||p|op||| 00| 0 eng d | ||
| 022 | _a1836-0947 | ||
| 022 | _a1836-5795 (Online) | ||
| 024 | 8 | _ahttps://doi.org/10.1071/CP10241 | |
| 040 | _aMX-TxCIM | ||
| 041 | _aeng | ||
| 090 | _aCIS-6117 | ||
| 100 | 0 |
_aXinxia Sui _95950 |
|
| 245 | 1 | 0 | _aDevelopment of an allele-specific marker for Glu-B3 alleles in common wheat and establishment of a multiplex PCR assay |
| 260 |
_aVictoria (Australia) : _bCSIRO Publishing, _c2010. |
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| 500 | _aPeer review | ||
| 500 | _aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=1836-0947 | ||
| 520 | _aLow-molecular-weight glutenin subunits (LMW-GS) have significant effects on the processing quality of end-use products of common wheat. It is more efficient to discriminate LMW-GS alleles with PCR-based molecular markers than with SDS-PAGE. In the present study, we developed an allele-specific PCR marker, designated Glu-B3abefg, which can be used to discriminate protein alleles Glu-B3a, b, e, f, and g simultaneously. Based on ten previously developed allele-specific STS markers, three multiplex PCRs, viz. Glu-B3c + Glu-B3d, Glu-B3b + Glu-B3g, and Glu-B3h + Glu-B3i, were established. Six Glu-B3 alleles (b, c, d, g, h, and i) could be discriminated using the three multiplex PCRs. Results of tests on 158 wheat varieties and lines using Glu-B3abefg and the three multiplex PCRs were consistent with those using the ten STS markers separately. The new allele-specific marker and three multiplex PCRs represent an efficient way to undertake marker-assisted selection of Glu-B3 alleles. | ||
| 536 | _aGlobal Wheat Program | ||
| 546 | _aText in English | ||
| 591 | _aCSIRO|CSIRO | ||
| 594 | _aINT2411 | ||
| 650 | 7 |
_aGenetic markers _2AGROVOC _91848 |
|
| 650 | 7 |
_aGlutenins _2AGROVOC _91137 |
|
| 650 | 7 |
_aPCR _2AGROVOC _912563 |
|
| 650 | 7 |
_aTriticum aestivum _2AGROVOC _91296 |
|
| 700 | 0 |
_aLin-hai Wang _920836 |
|
| 700 | 0 |
_aXianchun Xia _9377 |
|
| 700 | 0 |
_aZhenlin Wang _920926 |
|
| 700 | 1 |
_aHe Zhonghu _gGlobal Wheat Program _8INT2411 _9838 |
|
| 773 | 0 |
_tCrop and Pasture Science _gv. 61, no. 12, p. 978-987 _dVictoria (Australia) : CSIRO Publishing, 2010. _wG91570 _x1836-0947 |
|
| 856 | 4 |
_yAccess only for CIMMYT Staff _uhttps://hdl.handle.net/20.500.12665/792 |
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| 942 |
_cJA _2ddc _n0 |
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| 999 |
_c28273 _d28273 |
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