000			03159nab a22004457a 4500
001			G94470
003			MX-TxCIM
005			20230731182845.0
008			210805t2011 gw |||p|op||| 00| 0 eng d
022			_a1432-2242 (Online)
022	0		_a0040-5752
024	8		_ahttps://doi.org/10.1007/s00122-010-1490-7
040			_aMX-TxCIM
041			_aeng
090			_aCIS-6255
100	1		_9875 _aBABU, R. _gGlobal Maize Program _8INT2925
245	1	0	_aIsolation, fine mapping and expression profiling of a lesion mimic genotype, splNF4050-8 that confers blast resistance in rice
260			_aBerlin (Germany) : _bSpringer, _c2011.
500			_aPeer review
500			_aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0040-5752
520			_aWe evaluated a large collection of Tos17 mutant panel lines for their reaction to three different races of Magnaporthe oryzae and identified a lesion mimic mutant, NF4050-8, that showed lesions similar to naturally occurring spl5 mutant and enhanced resistance to all the three blast races tested. Nested modified-AFLP using Tos17-specific primers and southern hybridization experiments of segregating individuals indicated that the lesion mimic phenotype in NF4050-8 is most likely due to a nucleotide change acquired during the culturing process and not due to Tos17 insertion per se. Inheritance and genetic analyses in two japonica × indica populations identified an overlapping genomic region of 13 cM on short arm of chromosome 7 that was linked with the lesion mimic phenotype. High-resolution genetic mapping using 950 F3 and 3,821 F4 plants of NF4050-8 × CO39 delimited a 35 kb region flanked by NBARC1 (5.262 Mb) and RM8262 (5.297 Mb), which contained 6 ORFs; 3 of them were ‘resistance gene related’ with typical NBS–LRR signatures. One of them harbored a NB–ARC domain, which had been previously demonstrated to be associated with cell death in animals. Microarray analysis of NF4050-8 revealed significant up-regulation of numerous defense/pathogenesis-related genes and down-regulation of heme peroxidase genes. Real-time PCR analysis of WRKY45 and PR1b genes suggested possible constitutive activation of a defense signaling pathway downstream of salicylic acid but independent of NH1 in these mutant lines of rice.
536			_aGlobal Maize Program
546			_aText in English
591			_aSpringer
594			_aINT2925
650		7	_2AGROVOC _91243 _aRice
650		7	_2AGROVOC _92084 _aChromosome mapping
650		7	_2AGROVOC _93421 _aBlasts (of plants)
650		7	_2AGROVOC _92277 _aGenetic resistance
700	0		_aChang-Jie Jiang _921875
700	0		_aXin Xu _921876
700	1		_aKottapalli, K.R. _921877
700	0		_aHiroshi Takatsuji _921878
700	0		_aAkio Miyao _921879
700	0		_aHirohiko Hirochika _921880
700	0		_aShinji Kawasaki _94170
773	0		_tTheoretical and Applied Genetics _gv. 122, no. 4, p. 831-854 _dBerlin (Germany) : Springer, 2011. _w55521 _x0040-5752
856	4		_yAccess only for CIMMYT Staff _uhttps://hdl.handle.net/20.500.12665/294
942			_cJA _2ddc _n0
999			_c28250 _d28250