000 | 02859nab a22004097a 4500 | ||
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001 | G94064 | ||
003 | MX-TxCIM | ||
005 | 20230724204212.0 | ||
008 | 211110s2010 sa |||p|op||| 00| 0 eng d | ||
022 | _a1021-3589 | ||
022 | _a2224-8854 (Online) | ||
024 | 8 | _ahttps://doi.org/10.4001/003.018.0102 | |
040 | _aMX-TxCIM | ||
041 | _aeng | ||
090 | _aCIS-6039 | ||
100 | 1 |
_9473 _aDerera, J. |
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245 | 1 | 0 | _aAppraisal of protocol for the rapid screening of maize genotypes for maize weevil resistance |
260 |
_aSouth Africa : _bEntomological Society of Southern Africa, _c2010. |
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500 | _aPeer review | ||
500 | _aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=1021-3589 | ||
520 | _aA simplified, rapid protocol for screening maize genotypes for grain weevil resistance is required, therefore some modifications to the standard method (Dobie 1974) were appraised. The optimum oviposition period and minimum number of randomly selected weevils (Sitophilus zeamais Motschulsky) from a laboratory culture (without the procedure of sexing) to achieve similar progeny emergence to the standard method were determined under laboratory conditions. While results confirmed the effectiveness of the standard method in discriminating genotypes according to resistance, some modifications significantly reducing labour and level of skill required in implementing a rapid screening protocol were identified. Sex ratios in random samples of 10?50 weevils from a large laboratory culture were not significantly different from the 1:1 expected ratio (P >0.05) and therefore sexing of the populations was discarded. Infestation of grain with 32?50 weevils resulted in progeny emergence and kernel damage similar to the standard. Therefore, 32 weevils were recommended to infest grain for 10 days, without prior conditioning of insects to the test grain. Standard control hybrid varieties were also effectively validated. The modified procedure took 45?56 days to complete compared to about 90 days for the standard and did not sacrifice selection efficiency. | ||
536 | _aGenetic Resources Program | ||
546 | _aText in English | ||
594 | _aINT1617 | ||
595 | _aCSC | ||
650 | 7 |
_aCurculionidae _92535 _2AGROVOC |
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650 | 7 |
_aGrain _91138 _2AGROVOC |
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650 | 7 |
_aPest resistance _91199 _2AGROVOC |
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650 | 7 |
_aOviposition _918232 _2AGROVOC |
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650 | 7 |
_aScreening _95621 _2AGROVOC |
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650 | 7 |
_aSitophilus zeamais _92534 _2AGROVOC |
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700 | 1 |
_9832 _aPixley, K.V. _gGenetic Resources Program _8INT1617 |
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700 | 1 |
_aGiga, D.P. _994 |
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773 | 0 |
_tAfrican Entomology _gv. 18, no. 1, p. 8-16 _x1021-3589 _dSouth Africa : Entomological Society of Southern Africa, 2010. |
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856 | 4 |
_uhttps://hdl.handle.net/20.500.12665/933 _yAccess only for CIMMYT Staff |
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942 |
_cJA _2ddc _n0 |
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999 |
_c28073 _d28073 |