000 02859nab a22004097a 4500
001 G94064
003 MX-TxCIM
005 20230724204212.0
008 211110s2010 sa |||p|op||| 00| 0 eng d
022 _a1021-3589
022 _a2224-8854 (Online)
024 8 _ahttps://doi.org/10.4001/003.018.0102
040 _aMX-TxCIM
041 _aeng
090 _aCIS-6039
100 1 _9473
_aDerera, J.
245 1 0 _aAppraisal of protocol for the rapid screening of maize genotypes for maize weevil resistance
260 _aSouth Africa :
_bEntomological Society of Southern Africa,
_c2010.
500 _aPeer review
500 _aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=1021-3589
520 _aA simplified, rapid protocol for screening maize genotypes for grain weevil resistance is required, therefore some modifications to the standard method (Dobie 1974) were appraised. The optimum oviposition period and minimum number of randomly selected weevils (Sitophilus zeamais Motschulsky) from a laboratory culture (without the procedure of sexing) to achieve similar progeny emergence to the standard method were determined under laboratory conditions. While results confirmed the effectiveness of the standard method in discriminating genotypes according to resistance, some modifications significantly reducing labour and level of skill required in implementing a rapid screening protocol were identified. Sex ratios in random samples of 10?50 weevils from a large laboratory culture were not significantly different from the 1:1 expected ratio (P >0.05) and therefore sexing of the populations was discarded. Infestation of grain with 32?50 weevils resulted in progeny emergence and kernel damage similar to the standard. Therefore, 32 weevils were recommended to infest grain for 10 days, without prior conditioning of insects to the test grain. Standard control hybrid varieties were also effectively validated. The modified procedure took 45?56 days to complete compared to about 90 days for the standard and did not sacrifice selection efficiency.
536 _aGenetic Resources Program
546 _aText in English
594 _aINT1617
595 _aCSC
650 7 _aCurculionidae
_92535
_2AGROVOC
650 7 _aGrain
_91138
_2AGROVOC
650 7 _aPest resistance
_91199
_2AGROVOC
650 7 _aOviposition
_918232
_2AGROVOC
650 7 _aScreening
_95621
_2AGROVOC
650 7 _aSitophilus zeamais
_92534
_2AGROVOC
700 1 _9832
_aPixley, K.V.
_gGenetic Resources Program
_8INT1617
700 1 _aGiga, D.P.
_994
773 0 _tAfrican Entomology
_gv. 18, no. 1, p. 8-16
_x1021-3589
_dSouth Africa : Entomological Society of Southern Africa, 2010.
856 4 _uhttps://hdl.handle.net/20.500.12665/933
_yAccess only for CIMMYT Staff
942 _cJA
_2ddc
_n0
999 _c28073
_d28073