| 000 | 02664nab a22004097a 4500 | ||
|---|---|---|---|
| 001 | G93799 | ||
| 003 | MX-TxCIM | ||
| 005 | 20230811185610.0 | ||
| 008 | 210706s2010 xxu|||p|op||| 00| 0 eng d | ||
| 022 | _a0733-5210 | ||
| 024 | 8 | _ahttps://doi.org/10.1016/j.jcs.2010.01.005 | |
| 040 | _aMX-TxCIM | ||
| 041 | _aeng | ||
| 090 | _aCIS-5882 | ||
| 100 | 0 |
_aLin-hai Wang _920836 |
|
| 245 | 1 | 0 | _aDevelopment of STS markers and establishment of multiplex PCR for Glu-A3 alleles in common wheat (Triticum aestivum L.) |
| 260 |
_aUSA : _bElsevier, _c2010. |
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| 500 | _aPeer review | ||
| 500 | _aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0733-5210 | ||
| 520 | _aLow-molecular-weight glutenin subunits (LMW-GS) play a key role in determining the processing quality of the end-use products of common wheat. The objectives of this study were to identify genes at Glu-A3 locus, develop the STS markers, and establish multiplex PCR with the STS markers for Glu-A3 alleles. Gene-specific PCR primers were designed to amplify six near-isogenic lines (NILs) and Glenlea with different Glu-A3 alleles (a, b, c, d, e, f and g) defined by the protein electrophoretic mobility. Three Glu-A3 genes with complete coding sequence were cloned, designated as GluA3-1, GluA3-2 and GluA3-3, respectively. Seven dominant allele-specific STS (sequence tagged sites) markers were designed based on the SNPs (single nucleotide polymorphisms) among different allelic variants for the discrimination of the Glu-A3 protein alleles a, b, c, d, e, f and g. Four multiplex PCRs were established including Glu-A3b + Glu-A3f, Glu-A3d + Glu-A3f, Glu-A3d + Glu-A3g, and Glu-A3b + Glu-A3e. These markers and multiplex-PCR systems were validated on 141 CIMMYT wheat varieties and advanced lines with different Glu-A3 alleles, confirming that they can be efficiently used in marker-assisted breeding. | ||
| 536 | _aGlobal Wheat Program | ||
| 546 | _aText in English | ||
| 591 | _aElsevier | ||
| 594 | _aINT2411|INT0368 | ||
| 595 | _aCSC | ||
| 650 | 7 |
_aWheat _2AGROVOC _91310 |
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| 650 | 7 |
_aGlutenins _2AGROVOC _91137 |
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| 650 | 7 |
_aGenetic markers _2AGROVOC _91848 |
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| 650 | 7 |
_aPCR _2AGROVOC _912563 |
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| 700 | 0 |
_aGenying Li _95949 |
|
| 700 | 1 |
_aPeña-Bautista, R.J. _8INT0368 _gGlobal Wheat Program _9645 |
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| 700 | 0 |
_aXianchun Xia _9377 |
|
| 700 | 1 |
_aHe Zhonghu _gGlobal Wheat Program _8INT2411 _9838 |
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| 773 | 0 |
_tJournal of Cereal Science _gv. 51, no. 3, p. 305-312 _dUSA : Elsevier, 2010. _wG444514 _x0733-5210 |
|
| 856 | 4 |
_uhttps://hdl.handle.net/20.500.12665/583 _yAccess only for CIMMYT Staff |
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| 942 |
_cJA _2ddc _n0 |
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| 999 |
_c27969 _d27969 |
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