000			03038nab a22004097a 4500
001			G93469
003			MX-TxCIM
005			20230818175059.0
008			210705s2009 cc |||p|op||| 00| 0 eng d
022			_a0578-1752
024	8		_ahttps://doi.org/10.3864/j.issn.0578-1752.2009.01.003
040			_aMX-TxCIM
041			_aeng
090			_aCIS-5618
100	0		_aDan Liang _920831
245	1	0	_aCharacterization of Lr34/Yr18, Rht-B1b, Rht-D1b genes in CIMMYT wheat cultivars and advanced lines using STS markers
260			_aBeijing (China) : _bAcademy of Agricultural Sciences, _c2009.
500			_aPeer review
500			_aAbstrac in Chinese and English
500			_aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0578-1752
520			_a(Objective) Characterization of genes Lr34/Yr18, Rht-B1b (Rht1) and Rht-D1b (Rht2) in 263 CIMMYT wheat cultivars and advanced lines will benefit the improvement of rust resistance and plant height in Chinese wheat breeding program. (Method) A total of 263 CIMMYT wheat cultivars and advanced lines were tested by STS markers to understand the distribution of resistance genes Lr34/Yr18 and dwarfing genes Rht-B1b and Rht-D1b. (Result) The marker csLV34 could amplify a 150 bp fragment in the lines with Lr34/Yr18, and a 229 bp fragment in those lines without Lr34/Yr18. Two complementary markers, NH-BF.2/WR1.2 and NH-BF.2/MR1, were used to investigate the alleles Rht-B1a and Rht-B1b, respectively. They could amplify a 400 bp fragment in the genotypes with Rht-B1a and Rht-B1b, respectively. The marker DF/MR2 could generate a 280 bp fragment in the genotype with Rht-D1b. Of the 263 lines, 57 generated a 150 bp fragment with the marker csLV34, indicating the presence of Lr34/Yr18 in these lines, with a frequency of 21.7%. Two hundred and sixteen lines (82.1%) were detected to have the allele Rht-B1b and 38 lines (14.4%) contained the allele Rht-D1b. Twenty-one lines were detected to possess both the wild-type alleles Rht-B1a and Rht-D1a, while 12 had two dwarfing genes Rht-B1b and Rht-D1b. (Conclusion) These STS markers could be useful to detect the genes Lr34/Yr18, Rht-B1b and Rht-D1b. The frequency of Rht-B1b was much higher than that of Rht-D1b in CIMMYT lines.
536			_aGlobal Wheat Program
546			_aText in Chinese
594			_aINT2411
650		7	_aTriticum aestivum _2AGROVOC _91296
650		7	_aRusts _2AGROVOC _91251
650		7	_aDisease resistance _2AGROVOC _91077
650		7	_aGenes _2AGROVOC _93563
650		7	_aGenetic markers _2AGROVOC _91848
700	0		_aYang Fang-Ping _916892
700	1		_aHe Zhonghu _gGlobal Wheat Program _8INT2411 _9838
700	0		_919599 _aYao Da-Nian
700	0		_9377 _aXianchun Xia
773	0		_tScientia Agricultura Sinica _gv. 42, no. 1, p. 17-27 _dBeijing (China) : Academy of Agricultural Sciences, 2009. _wG445218 _x0578-1752
856	4		_yAccess only for CIMMYT Staff _uhttps://hdl.handle.net/20.500.12665/619
942			_cJA _2ddc _n0
999			_c27875 _d27875